采用超临界流体萃取法从济生肾气丸中提取丹皮酚。

Extraction of paeonol from Jisheng Shenqi Wan using supercritical fluid extraction.

作者信息

Liu Ben, Chang Yiling, Jiang Hailiang, Shen Bo

机构信息

Department of Bioengineering and Pharmaceutics, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, People's Republic of China.

出版信息

Biomed Chromatogr. 2007 Jan;21(1):79-83. doi: 10.1002/bmc.721.

DOI:10.1002/bmc.721

PMID:17080508

Abstract

Supercritical carbon dioxide with or without methanol and 95% ethanol modifiers was used to extract paeonol from the pills of Jisheng Shenqi Wan and high-performance liquid chromatography (HPLC) was used to analyze the extract. The effect of temperature and pressure on the yield of paeonol was tested. The results show that the recovery of paeonol was improved by adding a polar modifier, such as methanol and 95% ethanol in supercritical fluid. The yield obtained after 120 min extraction with 95% ethanol modified-supercritical carbon dioxide was the highest (1.51, w/w at 60 degrees C and 400 bar), while that obtained with supercritical carbon dioxide only at 40 degrees C was the lowest from 0.67 to 0.83 over a range of 200-600 bar.

摘要

使用含有或不含有甲醇和95%乙醇改性剂的超临界二氧化碳从济生肾气丸药丸中提取丹皮酚，并采用高效液相色谱法（HPLC）对提取物进行分析。测试了温度和压力对丹皮酚产率的影响。结果表明，在超临界流体中添加极性改性剂（如甲醇和95%乙醇）可提高丹皮酚的回收率。用95%乙醇改性的超临界二氧化碳萃取120分钟后获得的产率最高（在60℃和400巴时为1.51，w/w），而仅在40℃下使用超临界二氧化碳在200 - 600巴范围内获得的产率最低，为0.67至0.83。