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性激素结合球蛋白(SHBG)在人卵巢卵泡和黄体中的免疫定位。

Immunolocalization of sex hormone-binding globulin (SHBG) in human ovarian follicles and corpus luteum.

作者信息

Forges T, Gérard A, Monnier-Barbarino P, Gérard H

机构信息

Centre d'Assistance Médicale à la Procréation, Maternité Régionale et Universitaire, 10, rue du Dr Heydenreich, BP 4213, 54042 Nancy Cédex, France.

出版信息

Histochem Cell Biol. 2005 Sep;124(3-4):285-90. doi: 10.1007/s00418-005-0006-z. Epub 2005 Oct 28.

Abstract

Sex hormone-binding globulin (SHBG), a hepatic carrier protein for sex steroids is expressed in different steroid-sensitive tissues, including Sertoli cells of the testis. It has been suggested that this protein may be one of the local regulators of spermatogenesis. The expression of SHBG in the ovary is currently unknown. We have previously demonstrated the synthesis of SHBG in granulosa-lutein cells from patients undergoing in vitro fertilization. In this study, the presence of SHBG in human ovarian follicles and corpora lutea is investigated, using immunohistochemistry on adult and fetal ovarian tissue sections. SHBG is localized in the whole granulosa layer at all stages of folliculogenesis, whereas, only isolated theca cells are immunostained. In primordial and primary follicles, the oocyte cytoplasm shows an intense immunostaining, which disappears after the secondary stage. In the microenvironment of the mature oocytes, SHBG is present in the surrounding cumulus cells, the perivitelline space, and nearby the oolemma. In the corpus luteum, SHBG is localized in large luteal cells, whereas, small luteal cells do not show any significant staining. By analogy with the testis, these results raise the question of an involvement of SHBG in the regulation of follicular maturation as well as in luteal function.

摘要

性激素结合球蛋白(SHBG)是一种肝脏分泌的性类固醇载体蛋白,在包括睾丸支持细胞在内的不同类固醇敏感组织中表达。有人提出,这种蛋白质可能是精子发生的局部调节因子之一。目前尚不清楚SHBG在卵巢中的表达情况。我们之前已经证明,接受体外受精的患者的颗粒黄体细胞能够合成SHBG。在本研究中,通过对成人和胎儿卵巢组织切片进行免疫组织化学,研究了人卵巢卵泡和黄体中SHBG的存在情况。在卵泡发生的各个阶段,SHBG定位于整个颗粒层,而只有孤立的卵泡膜细胞被免疫染色。在原始卵泡和初级卵泡中,卵母细胞细胞质显示出强烈的免疫染色,在次级阶段后消失。在成熟卵母细胞的微环境中,SHBG存在于周围的卵丘细胞、卵周间隙和卵膜附近。在黄体中,SHBG定位于大黄体细胞,而小黄体细胞没有明显的染色。与睾丸类似,这些结果提出了SHBG参与卵泡成熟调节以及黄体功能调节的问题。

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