Forges T, Gérard A, Hess K, Monnier-Barbarino P, Gérard H
Centre d/Assistance Médicale à la Procréation, Maternité Régionale et Universitaire, Nancy, France.
Mol Cell Endocrinol. 2004 Apr 30;219(1-2):61-8. doi: 10.1016/j.mce.2004.01.011.
Sex hormone-binding globulin (SHBG) was classically thought to be a plasma steroid-carrying protein of hepatic origin, but recently, locally produced, membrane-bound SHBG has been shown to influence cell functions in several steroid-responsive tissues. In the ovary, SHBG is known to be present in the follicular fluid, but information about a possible intracellular presence of SHBG in this organ is still very scarce. In this study the presence of SHBG was assessed by immunohistochemistry in human granulosa-lutein cells (GLC) collected by follicle puncture for in vitro fertilization. SHBG was detected in the cytoplasm of GLC before and after in vitro culture for up to 96 h. The presence of full-length SHBG messenger RNA was demonstrated in GLC by reverse transcription-polymerase chain reaction (RT-PCR) in both cultured and uncultured cells. These results demonstrate a local synthesis of SHBG in GLC and raise the question of the physiological significance of these findings in follicular physiology.
性激素结合球蛋白（SHBG）传统上被认为是一种源自肝脏的血浆类固醇运载蛋白，但最近研究表明，局部产生的膜结合SHBG会影响几种类固醇反应性组织中的细胞功能。在卵巢中，已知卵泡液中存在SHBG，但关于该器官中SHBG可能存在于细胞内的信息仍然非常稀少。在本研究中，通过免疫组织化学方法评估了通过卵泡穿刺收集用于体外受精的人颗粒黄体细胞（GLC）中SHBG的存在情况。在体外培养长达96小时前后的GLC细胞质中均检测到了SHBG。通过逆转录聚合酶链反应（RT-PCR）在培养和未培养的细胞中均证实了GLC中存在全长SHBG信使RNA。这些结果证明了GLC中SHBG的局部合成，并提出了这些发现在卵泡生理学中的生理意义问题。
