Wise Kimberly C, Manna Sunil K, Yamauchi Keiko, Ramesh Vani, Wilson Bobby L, Thomas Renard L, Sarkar Shubhashish, Kulkarni Anil D, Pellis Neil R, Ramesh Govindarajan T
Molecular Neurotoxicology Laboratory, Department of Biology, Texas Southern University, Houston, Texas 77004, USA.
In Vitro Cell Dev Biol Anim. 2005 Mar-Apr;41(3-4):118-23. doi: 10.1290/0501006.1.
Microgravity induces inflammatory responses and modulates immune functions that may increase oxidative stress. Exposure to a microgravity environment induces adverse neurological effects; however, there is little research exploring the etiology of these effects resulting from exposure to such an environment. It is also known that spaceflight is associated with increase in oxidative stress; however, this phenomenon has not been reproduced in land-based simulated microgravity models. In this study, an attempt has been made to show the induction of reactive oxygen species (ROS) in mice brain, using ground-based microgravity simulator. Increased ROS was observed in brain stem and frontal cortex with concomitant decrease in glutathione, on exposing mice to simulated microgravity for 7 d. Oxidative stress-induced activation of nuclear factor-kappaB was observed in all the regions of the brain. Moreover, mitogen-activated protein kinase kinase was phosphorylated equally in all regions of the brain exposed to simulated microgravity. These results suggest that exposure of brain to simulated microgravity can induce expression of certain transcription factors, and these have been earlier argued to be oxidative stress dependent.
微重力会引发炎症反应并调节免疫功能，这可能会增加氧化应激。暴露于微重力环境会引发不良的神经学效应；然而，对于暴露于这种环境所导致的这些效应的病因，几乎没有相关研究。众所周知，太空飞行与氧化应激的增加有关；然而，这种现象在地面模拟微重力模型中尚未得到重现。在本研究中，尝试使用地面微重力模拟器来展示小鼠大脑中活性氧（ROS）的诱导情况。将小鼠暴露于模拟微重力环境7天后，在脑干和额叶皮质中观察到ROS增加，同时谷胱甘肽减少。在大脑的所有区域均观察到氧化应激诱导的核因子-κB激活。此外，在暴露于模拟微重力的大脑所有区域中，丝裂原活化蛋白激酶激酶均被同等程度地磷酸化。这些结果表明，大脑暴露于模拟微重力可诱导某些转录因子的表达，而这些转录因子此前被认为是依赖于氧化应激的。
