Arroyo R, Engbring J, Alderete J F
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758.
Mol Microbiol. 1992 Apr;6(7):853-62. doi: 10.1111/j.1365-2958.1992.tb01536.x.
Parasitism of host epithelial cells by Trichomonas vaginalis is a highly specific event. Four trichomonad surface proteins (adhesins) with molecular masses of 65,000 daltons (65 kDa; AP65), 51 kDa (AP51), 33 kDa (AP33), and 23 kDa (AP23) mediate the interaction of T. vaginalis with epithelial cells. Fresh isolates, when compared with long-term-grown isolates, had greater amounts of adhesins, which corresponded with increased levels of cytoadherence. Anti-adhesin antibodies reacted by immunoblot only with the respective protein and detected, by indirect immunofluorescence, each adhesion on the parasite surface. These antibodies inhibited the binding of live parasites to epithelial cells and protected epithelial cells from contact-dependent cytotoxicity. The pretreatment of epithelial cells with a preparation of purified adhesions also blocked trichomonal cytoadherence. Moreover, HeLa cells possessed molecules which recognized and bound to adhesins on nitrocellulose blots.
阴道毛滴虫对宿主上皮细胞的寄生是一个高度特异性的事件。四种分子量分别为65,000道尔顿（65 kDa；AP65）、51 kDa（AP51）、33 kDa（AP33）和23 kDa（AP23）的滴虫表面蛋白（黏附素）介导了阴道毛滴虫与上皮细胞的相互作用。与长期培养的分离株相比，新鲜分离株具有更多的黏附素，这与细胞黏附水平的增加相对应。抗黏附素抗体通过免疫印迹仅与相应蛋白发生反应，并通过间接免疫荧光检测寄生虫表面的每种黏附素。这些抗体抑制活寄生虫与上皮细胞的结合，并保护上皮细胞免受接触依赖性细胞毒性的影响。用纯化黏附素制剂预处理上皮细胞也可阻断滴虫的细胞黏附。此外，HeLa细胞具有能识别并结合硝酸纤维素膜上黏附素的分子。
