Ioannidis Anastasios, Papaioannou Panagiota, Magiorkinis Emmanouil, Magana Maria, Ioannidou Vasiliki, Tzanetou Konstantina, Burriel Angeliki R, Tsironi Maria, Chatzipanagiotou Stylianos
Department of Nursing, Faculty of Human Movement and Quality of Life Sciences, University of PeloponneseSparta, Greece.
Department of Biopathology and Clinical Microbiology, Athens Medical School, Aeginition HospitalAthens, Greece.
Front Microbiol. 2017 Jun 28;8:1188. doi: 10.3389/fmicb.2017.01188. eCollection 2017.
The symbiosis of and is the first described association between two obligate human parasites. is the niche and the vector for the transmission of infection. This clinically significant symbiosis may affect virulence and susceptibility to treatment. The aims of this study were to investigate the intracellularly present and species in strains isolated from the vaginal discharge of infected women as well as to trace the diversity pattern among the species detected in the isolated strains. Hundred pure cultures were isolated from ~7,500 patient specimens presented with clinical purulent vaginitis. PCR and sequencing for spp. were performed in DNA extracted from the pure cultures. In addition, vaginal discharge samples were cultured for the presence of and . Phylogenetic analysis assisted the identification of interspecies relationships between the and isolates. Fifty four percentage of isolates were harboring spp. Phylogenetic analysis revealed three distinct clusters, two with already characterized and spp. (37% of total spp.), whereas one group formed a distinct cluster matched with the newly identified species (59.3%) and one or more unknown spp. (3.7%). strains associated with vaginal infection might host intracellular mycoplasmas or ureaplasmas. Intracellular Mollicutes that remain undetected in the extracellular environment when conventional diagnostic methods are implemented may comprise either novel species, such as , or unknown species with yet unexplored clinical significance.
[某种微生物]与[另一种微生物]的共生关系是首次描述的两种专性人体寄生虫之间的关联。[某种微生物]是[另一种微生物]感染的生态位和传播载体。这种具有临床意义的共生关系可能会影响[另一种微生物]的毒力和对治疗的敏感性。本研究的目的是调查从感染女性阴道分泌物中分离出的[某种微生物]菌株细胞内存在的[某种微生物]和脲原体种类，并追踪分离菌株中检测到的物种之间的多样性模式。从约7500例临床脓性阴道炎患者标本中分离出100株纯[某种微生物]培养物。对从纯培养物中提取的DNA进行[某种微生物]属的PCR和测序。此外，对阴道分泌物样本进行培养以检测[某种微生物]和脲原体的存在。系统发育分析有助于确定[某种微生物]和脲原体分离株之间的种间关系。54%的[某种微生物]分离株携带脲原体属。系统发育分析揭示了三个不同的聚类，其中两个聚类包含已鉴定的[某种微生物]和脲原体属物种（占脲原体属总数的37%），而一组形成了一个与新鉴定的物种[某种微生物]（59.3%）和一种或多种未知脲原体属物种（3.7%）匹配的独特聚类。与阴道感染相关的[某种微生物]菌株可能携带细胞内支原体或脲原体。当采用传统诊断方法时，在细胞外环境中未检测到的细胞内柔膜菌可能包括新物种，如[某种微生物]，或具有尚未探索的临床意义的未知物种。
