Johannes C, Boes R, Obe G
University GH Essen, Department of Genetics, FRG.
Mutagenesis. 1992 May;7(3):225-32. doi: 10.1093/mutage/7.3.225.
Chinese hamster ovary cells were exposed to the restriction endonuclease AluI in the presence of 2.2 M glycerol or 0.9 M sorbitol using different treatment regimens. Induced chromosomal aberrations were taken as indication for cellular uptake of AluI. The results indicate that cellular uptake of AluI is mediated by vesicles formed during transition of hypertonic to isotonic conditions. Addition of medium leads to breakdown of these vesicles and to liberation of AluI molecules which eventually may reach the chromatin.
使用不同的处理方案，将中国仓鼠卵巢细胞在2.2 M甘油或0.9 M山梨醇存在的情况下暴露于限制性内切酶AluI。诱导的染色体畸变被用作AluI细胞摄取的指标。结果表明，AluI的细胞摄取是由高渗到等渗条件转变过程中形成的囊泡介导的。添加培养基会导致这些囊泡破裂，并释放出AluI分子，这些分子最终可能会到达染色质。
