Obe G, Johannes C, Werthmann I, Folle G A
Department of Genetics, Universität GH Essen, Germany.
Mutat Res. 1993 May;299(3-4):305-11. doi: 10.1016/0165-1218(93)90107-o.
Treatment of CHO cells with AluI in the S-phase leads to chromatid-type aberrations whose frequencies are linearly correlated with dose. Treatment in the S-phase leads to fewer aberrations than treatment in the G1-phase, which is comparable to chromosomal aberration induction following X-irradiation in the G1- and S-phases. Treatment in the G1-phase leads to few chromatid-type interchanges, some of these may originate from DNA single-strand gaps induced by AluI in canonical structures of DNA and in DNA.RNA hybrids.
在S期用AluI处理CHO细胞会导致染色单体型畸变，其频率与剂量呈线性相关。在S期处理产生的畸变比在G1期处理少，这与在G1期和S期进行X射线照射后诱导的染色体畸变相当。在G1期处理导致很少的染色单体型互换，其中一些可能源于AluI在DNA的经典结构和DNA.RNA杂交体中诱导的DNA单链缺口。
