Chen L M, Cui Y H, Qin M, Wang Y L, Bai X L, Ma Q S
Laboratory of Molecular Genetics, Guangxi Agricultural College, Nanning, China.
Mol Gen Genet. 1992 May;233(1-2):311-4. doi: 10.1007/BF00587593.
Clones from a Frankia At4 gene bank were pooled into groups and mass conjugated into a nodD mutant of Rhizobium leguminosarum bv. viciae by triparental matings. When peas were inoculated with the pooled transconjugants, nodulation was observed. A plasmid, pAt2GX containing Frankia DNA, was isolated from bacteria recovered from these nodules. This plasmid was shown to complement a nodD mutant of R. leguminosarum bv. viciae. Thus pAt2GX contains a Frankia gene that is functionally equivalent to nodD of R. leguminosarum bv. viciae.
将来自弗兰克氏菌At4基因文库的克隆分组汇集，通过三亲本杂交大量接合到豌豆根瘤菌蚕豆生物变种的nodD突变体中。当用汇集的转接合子接种豌豆时，观察到了结瘤现象。从这些根瘤中回收的细菌中分离出了一个含有弗兰克氏菌DNA的质粒pAt2GX。该质粒被证明可互补豌豆根瘤菌蚕豆生物变种的nodD突变体。因此，pAt2GX含有一个功能上等同于豌豆根瘤菌蚕豆生物变种nodD的弗兰克氏菌基因。
