Squartini A, van Veen R J, Regensburg-Tuink T, Hooykaas P J, Nuti M P
Dipartimento di Biotecnologie Agrarie, Università di Padova, Italy.
Mol Plant Microbe Interact. 1988 Mar;1(3):145-9. doi: 10.1094/mpmi-1-145.
A gene library of the symbiotic 240-kb plasmid of Rhizobium leguminosarum strain 1001 was constructed in pUC18. The clones showing homology with a 6.6-kb fragment containing nodEFDABC from the Sym plasmid pRLlJI were detected by colony hybridization. Additional probes from the symbiotic region of pRLlJI were used to localize the corresponding genes on the map of pRle1001a. The relative positions of nod and nif gene clusters are different than those of pRLlJI. A comparison of the amino acid sequence for NodD from pRle1001a with NodD proteins from other Rhizobium species showed a high degree of sequence conservation at the amino terminus of the protein.
构建了豌豆根瘤菌1001菌株共生的240kb质粒的基因文库，该文库构建于pUC18中。通过菌落杂交检测出与来自共生质粒pRLlJI的含nodEFDABC的6.6kb片段具有同源性的克隆。使用来自pRLlJI共生区域的其他探针将相应基因定位在pRle1001a图谱上。nod和nif基因簇的相对位置与pRLlJI的不同。将pRle1001a的NodD氨基酸序列与其他根瘤菌属物种的NodD蛋白进行比较，结果显示该蛋白氨基末端具有高度的序列保守性。
