de Lisle G W, Yates G F, Collins D M, MacKenzie R W, Crews K B, Walker R
AgResearch, Wallaceville Animal Research Centre, P.O. Box 40-063, Upper Hutt, New Zealand.
N Z Vet J. 1995 Dec;43(7):266-71. doi: 10.1080/00480169./1995.35905.
The MacKenzie Basin, an area of about 5150 km2 in the South Island of New Zealand, was free of bovine tuberculosis prior to 1980. During the next 13 years, the majority of the cattle and deer herds in this area became infected with Mycobacterium bovis. The history of infection in the MacKenzie Basin has all the characteristics of a newly developed region of endemic tuberculosis with a wildlife reservoir of M. bovis. Tuberculous possums and ferrets were found in the MacKenzie Basin and both may have been a source of infection for domestic animals. DNA fingerprinting of 125 isolates of M. bovis from domestic animals and wildlife by restriction endonuclease analysis revealed two major groups of isolates. The same groups were identified using IS6110 as a DNA probe. Restriction endonuclease analysis enabled one group to be subdivided into seven restriction types and the other group into eight types. Mycobacterium bovis isolates with the most common restriction types were present in both domestic animals and wildlife, indicating that infection had spread between these two groups of animals. DNA fingerprinting also revealed that M. bovis was introduced into the MacKenzie Basin from at least two distinct sources. Furthermore, DNA finger-printing was able to identify probable sources of infection.
麦肯齐盆地位于新西兰南岛，面积约5150平方公里，1980年前没有牛结核病。在接下来的13年里，该地区的大多数牛群和鹿群感染了牛分枝杆菌。麦肯齐盆地的感染史具有新出现的地方性结核病流行区的所有特征，存在牛分枝杆菌的野生动物宿主。在麦肯齐盆地发现了患结核病的负鼠和雪貂，它们都可能是家畜的感染源。通过限制性内切酶分析对来自家畜和野生动物的125株牛分枝杆菌进行DNA指纹图谱分析，发现了两个主要的菌株群。使用IS6110作为DNA探针也鉴定出了相同的菌群。限制性内切酶分析使一组可细分为七种限制性类型，另一组细分为八种类型。具有最常见限制性类型的牛分枝杆菌菌株在家畜和野生动物中均有出现，这表明感染在这两组动物之间传播。DNA指纹图谱分析还表明，牛分枝杆菌至少从两个不同来源传入麦肯齐盆地。此外，DNA指纹图谱分析能够确定可能的感染源。
