de Lisle Geoffrey W, Kawakami R Pamela, Yates Gary F, Collins Desmond M
AgResearch, National Centre for Biosecurity and Infectious Disease, Wallaceville, P.O. Box 40063, Upper Hutt, New Zealand.
Vet Microbiol. 2008 Dec 10;132(3-4):402-7. doi: 10.1016/j.vetmic.2008.05.022. Epub 2008 Jul 15.
As part of wildlife surveillance for bovine tuberculosis, pooled lymph nodes from 21,481 ferrets, 1056 stoats and 83 weasels were cultured for mycobacteria. A total of 268 isolates of Mycobacterium bovis were obtained from ferrets, 2 from stoats and none from weasels, demonstrating the presence of a wildlife reservoir of infection in ferrets. DNA typing by restriction endonuclease analysis (REA) of 48 selected isolates of M. bovis revealed 23 REA types. Twenty-one of these types had previously been isolated from cattle and farmed deer, demonstrating a complex cycle of infection involving wildlife and domestic animals. Apart from M. bovis, a further 208 mycobacterial isolates were obtained, the majority of which (178) were members of the M. avium complex. Speciation of the remaining 30 mycobacterial isolates by DNA sequencing of the 16s rRNA gene, identified half the isolates as M. triplex. Other species identified included M. fortuitum, M. florentinum, M. interjectum, M. intracellulare, M. holsaticum, and M. septicum/M. peregrinum.
作为牛结核病野生动物监测的一部分，对21481只雪貂、1056只白鼬和83只黄鼬的淋巴结进行混合培养，以检测分枝杆菌。从雪貂中总共获得了268株牛分枝杆菌分离株，从白鼬中获得了2株，从黄鼬中未获得，这表明雪貂中存在野生动物感染源。通过限制性内切酶分析（REA）对48株选定的牛分枝杆菌分离株进行DNA分型，发现了23种REA类型。其中21种类型以前曾从牛和养殖鹿中分离出来，这表明存在一个涉及野生动物和家畜的复杂感染循环。除牛分枝杆菌外，还获得了另外208株分枝杆菌分离株，其中大多数（178株）是鸟分枝杆菌复合体的成员。通过对16s rRNA基因进行DNA测序，对其余30株分枝杆菌分离株进行鉴定，发现一半的分离株为三联分枝杆菌。鉴定出的其他物种包括偶然分枝杆菌、佛罗伦萨分枝杆菌、插入分枝杆菌、胞内分枝杆菌、霍尔萨特分枝杆菌和败血分枝杆菌/漂泊分枝杆菌。
