Qiao H-L, Liu J-H, Yang J, Dong Z-M
Department of Clinical Pharmacology, School of Medicine, Zhengzhou University, Zhengzhou 450052, China.
Int J Clin Pract. 2005 Aug;59(8):895-9. doi: 10.1111/j.1742-1241.2005.00496.x.
The aim of this study was to investigate the relationships between skin test, specific immunoglobulin (Ig) E and cytokines in penicillin allergy. We collected the sera of 259 patients with historical positive skin test to penicillins, with immediate positive skin test and with a negative skin test results. The positive rate of specific IgE antibodies in 259 patients was 62.2% (161) by using radioallergosorbent test (RAST). Of the eight kinds of antigenic determinants, the positive rates of specific IgE to major and minor determinants were 43.63% (113) and 52.51% (136), respectively (p < 0.05). In 122 patients with immediate positive skin test, when the degrees of skin test were +, 2+, 3+ and 4+, the positive rates of specific IgE were 45.7, 57.1, 85.2 and 100%, respectively. The levels of interleukin (IL)-4, IL-13 and interferon (IFN)-gamma in the sera of patients with positive skin test were significantly increased with the degree of positive skin test (p < 0.05). The combined use of major and minor determinants in RAST offers the better test for the detection of penicillin-specific IgE antibodies. IL-4, IL-13 and IFN-gamma play important roles in penicillin allergy.
本研究旨在探讨皮肤试验、特异性免疫球蛋白（Ig）E和细胞因子在青霉素过敏中的关系。我们收集了259例青霉素皮肤试验历史阳性、即刻皮肤试验阳性和皮肤试验结果阴性患者的血清。采用放射变应原吸附试验（RAST）检测，259例患者中特异性IgE抗体阳性率为62.2%（161例）。在8种抗原决定簇中，特异性IgE对主要和次要决定簇的阳性率分别为43.63%（113例）和52.51%（136例）（p<0.05）。在122例即刻皮肤试验阳性患者中，当皮肤试验程度为+、2+、3+和4+时，特异性IgE阳性率分别为45.7%、57.1%、85.2%和100%。皮肤试验阳性患者血清中白细胞介素（IL）-4、IL-13和干扰素（IFN）-γ水平随皮肤试验阳性程度显著升高（p<0.05）。RAST中主要和次要决定簇联合使用对检测青霉素特异性IgE抗体的检测效果更好。IL-4、IL-13和IFN-γ在青霉素过敏中起重要作用。
