Qiao Hai-Ling, Wen Qiang, Gao Na, Tian Xin, Jia Lin-Jing
Department of Clinical Pharmacology, School of Medicine, Zhengzhou University, Zhengzhou, 450052, People's Republic of China.
Eur J Clin Pharmacol. 2007 Mar;63(3):263-9. doi: 10.1007/s00228-006-0245-5. Epub 2007 Jan 16.
Our aim was to investigate the hypothesis that the sera interleukin-10 (IL-10) level and polymorphic nucleotides within the IL-10 gene promoters would link to specific IgE and IgG production and the expression of penicillin allergy.
One hundred and two patients and 86 healthy subjects were chosen for assay of serum IL-10 level by enzyme-linked immunosorbent assay (ELISA) and type -1082 G/A and -819 C/T alleles by sequence-specific primer polymerase chain reaction (SSP-PCR). Radioallergosorbent test (RAST) and ELISA were used to examine eight types of specific immunoglobulin-E (IgE) and IgG antibodies, respectively, which included four types of antibodies to major and minor antigenic determinants.
Compared with control subjects and patients with negative-specific IgE, there were significantly lower levels of IL-10 in patients with positive-specific IgE (P < 0.05). Similarly, there were significantly lower levels of IL-10 in patients with positive-specific IgG compared with normal controls and allergic patients with negative-specific IgG (P < 0.05). The visible negative correlations existed between IL-10 and four types of specific IgE [benzylpenicilloyl (BPO), phenoxomethylpenicilloyl (PVO), benzylpenicillanyl (BPA), amoxicillanyl (AXA)], and patients with three or more positive-specific IgE had significantly lower IL-10 levels than normal controls (P < 0.01). There was a declining trend for IL-10 level in serum with the increase in types of positive-specific IgE. But there was no significant difference in serum IL-10 level between the positive skin-test group and the allergic-history group. Compared with controls and patients with negative antibodies, a significantly decreased frequency of the -1082 G allele was present in patients with positive antibodies (P < 0.01). The allele T and TT genotype at -819 C/T position had lower frequency in the negative-specific IgG group than that in the positive group and controls (P < 0.01).
Positive specific IgE and IgG are associated with decreased IL-10 level in allergic reaction to penicillins. The distributions of genotype and frequency of allele at the -1082 G/A position may be associated with the production of both specific IgE and IgG antibodies.
我们旨在研究白细胞介素-10（IL-10）血清水平及IL-10基因启动子内多态性核苷酸与特异性IgE和IgG产生以及青霉素过敏表达之间存在关联这一假说。
选取102例患者和86名健康受试者，采用酶联免疫吸附测定法（ELISA）检测血清IL-10水平，采用序列特异性引物聚合酶链反应（SSP-PCR）检测-1082 G/A和-819 C/T等位基因。分别采用放射变应原吸附试验（RAST）和ELISA检测8种特异性免疫球蛋白E（IgE）和IgG抗体，其中包括4种针对主要和次要抗原决定簇的抗体。
与对照组和特异性IgE阴性患者相比，特异性IgE阳性患者的IL-10水平显著降低（P < 0.05）。同样，与正常对照组和特异性IgG阴性的过敏患者相比，特异性IgG阳性患者的IL-10水平显著降低（P < 0.05）。IL-１０与4种特异性IgE［苄青霉素酰基（BPO）、苯氧甲基青霉素酰基（PVO）、苄青霉素基（BPA）、阿莫西林基（AXA）］之间存在明显的负相关，3种及以上特异性IgE阳性的患者IL-10水平显著低于正常对照组（P < 0.01）。随着特异性IgE阳性类型数量增加，血清中IL-10水平呈下降趋势。但阳性皮肤试验组和有过敏史组之间血清IL-10水平无显著差异。与对照组和抗体阴性患者相比，抗体阳性患者中-1082 G等位基因频率显著降低（P < 0.01）。在-819 C/T位点，等位基因T和TT基因型在特异性IgG阴性组中的频率低于阳性组和对照组（P < 0.01）。
在青霉素过敏反应中，阳性特异性IgE和IgG与IL-10水平降低有关。-1082 G/A位点的基因型分布和等位基因频率可能与特异性IgE和IgG抗体的产生均有关。
