Lo H-C, Chen Y-W, Chien C-H, Tseng C-Y, Kuo Y-M, Huang B-M
Department of Medical Education and Research, Changhua Christian Hospital, Changhua, Taiwan, Republic of China.
Arch Androl. 2005 Jul-Aug;51(4):285-94. doi: 10.1080/014850190923404.
Tremella mesenterica (TM), a yellow jelly mushroom, has been traditionally used as food and crude medicine to improve several kinds of symptoms in Chinese society for a long time. Recent studies have illustrated that the fractions of fruiting bodies of TM exhibit a significant hypoglycemic activity in diabetic mouse models, which usually suffer from sexual dysfunction. In a previous study, we showed that TM reduced plasma testosterone production in normal rats without any positive effect in diabetic rats. It evolved a question of TM directly regulating Leydig cell steroidogenesis. In this study, MA-10 mouse Leydig tumor cells were treated with vehicle, different dosages of TM with or without human chorionic gonadotropin (hCG 50 ng/ml) to clarify the effects. Results showed that TM at different dosages (0.01-10 mg/ml) did not have any effect on MA-10 cell steroidogenesis (p > 0.05). In the presence of hCG, there was an inhibitory trend that TA suppressed MA-10 cell progesterone production at 3 hr treatment with a statistically significant difference by the 10 mg/ml TM (p < 0.05). In time course effect, TM alone did not have any effect on MA-10 cell steroidogenesis from at 1, 2, 3, 6 and 12 hr (p > 0.05). However, TM did reduce hCG-treated MA-10 cell progesterone production at 1, 2 and 3 hr (p < 0.05), respectively. To determine whether TM would have adverse effects on MA-10 cell steroidogenesis in the presence of hCG, MTT assay and recovery studies were conducted. MTT assay indicated that TM had no effect on surviving cells. In addition, with the removal of TM, and then the addition of hCG (2 and 4 hr), progesterone levels were restored within 4 hr. Taken together, present studies suggested that TM suppressed hCG-treated steroidogenesis in MA-10 cells without any toxicity effect.
金耳（TM），一种黄色的胶质蘑菇，长期以来在中国社会一直被用作食物和草药来改善多种症状。最近的研究表明，金耳子实体的提取物在糖尿病小鼠模型中表现出显著的降血糖活性，而糖尿病小鼠通常会出现性功能障碍。在之前的一项研究中，我们发现金耳可降低正常大鼠的血浆睾酮水平，但对糖尿病大鼠没有任何积极作用。这就引出了金耳是否直接调节睾丸间质细胞类固醇生成的问题。在本研究中，用溶剂、不同剂量的金耳（有无人绒毛膜促性腺激素（hCG 50 ng/ml））处理MA-10小鼠睾丸间质细胞瘤细胞，以阐明其作用效果。结果显示，不同剂量（0.01 - 10 mg/ml）的金耳对MA-10细胞类固醇生成没有任何影响（p > 0.05）。在hCG存在的情况下，有一种抑制趋势，即10 mg/ml的金耳处理3小时可抑制MA-10细胞孕酮生成，差异具有统计学意义（p < 0.05）。在时间进程效应方面，单独的金耳在1、2、3、6和12小时对MA-10细胞类固醇生成没有任何影响（p > 0.05）。然而，金耳分别在1、2和3小时确实降低了hCG处理的MA-10细胞孕酮生成（p < 0.05）。为了确定在hCG存在的情况下金耳是否会对MA-10细胞类固醇生成产生不利影响，进行了MTT试验和恢复研究。MTT试验表明金耳对存活细胞没有影响。此外，去除金耳后再添加hCG（2和4小时），孕酮水平在4小时内恢复。综上所述，目前的研究表明金耳可抑制hCG处理的MA-10细胞类固醇生成，且没有任何毒性作用。
