Aahmat Yusup, Chen Tongyi, Chen Zhongwei, Liu Dapeng, Wang Zhenbin
Orthopedics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi Xinjiang, 830054, P. R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Jun;19(6):439-42.
To investigate the ability of the biodegradable polycaprolactone (PCL) material to repair bone defect and to evaluate the feasibility of using the PCL as the scaffold in tissue engineering bone.
The bone defect models of 4. 5 mm x 12 mm were made in the bilateral femoral condyle of 65 New Zealand white rabbits. The PCL cylinder was implanted into the right side of defect (experimental group, n = 60), the high dense hydroxyapatite was implanted into the left side of defect (control group, n = 60), and the incision was sutured without any implants (blank group, n = 5). The samples were harvested and observed by examinations of gross, X-ray, bone density, 99mTc-MDP bone scanning, gamma-display ratio and scanning electron microscope (SEM) after 3, 6, 9 and 12 months of operation. The results were compared between the experimental group and control group.
At 3, 6, 9 and 12 months after operation, the gross and X-ray examinations indicated that the bone defect filled with the new bone on the PCL-tissue surface, and no delayed inflammatory reaction appeared. The average bone mineral density was greater in the experimental group than that in the control group, and the difference had statistical significance (P< 0. 05). The results of 99mTc-MDP bone scanning and gamma-display ratio showed that the nuclide uptake was more in the PCL group than that in the control group. The SEM result proved that the new compact bone formed on the PCL migrating surface as the PCL degraded gradually,but the collagen fiber sheath formed around the hydroxyapatite in the control group.
PCL possesses good biocompatibility and high bone inductive potentiality, it can be used to repair bone defect.
研究可生物降解聚己内酯（PCL）材料修复骨缺损的能力，并评估其作为组织工程骨支架的可行性。
在65只新西兰白兔双侧股骨髁制备4.5 mm×12 mm的骨缺损模型。将PCL圆柱体植入缺损右侧（实验组，n = 60），将高密度羟基磷灰石植入缺损左侧（对照组，n = 60），切口缝合不植入任何材料（空白组，n = 5）。术后3、6、9和12个月取材，通过大体观察、X线、骨密度、99mTc-MDP骨扫描、γ显示率及扫描电子显微镜（SEM）观察，比较实验组与对照组结果。
术后3、6、9和12个月，大体及X线检查显示PCL组织表面骨缺损被新生骨填充，无延迟性炎症反应。实验组平均骨密度大于对照组，差异有统计学意义（P<0.05）。99mTc-MDP骨扫描及γ显示率结果表明PCL组核素摄取多于对照组。SEM结果证明随着PCL逐渐降解，在其迁移表面形成新的致密骨，而对照组羟基磷灰石周围形成胶原纤维鞘。
PCL具有良好的生物相容性和较高的骨诱导潜力，可用于修复骨缺损。
