Gredes Tomasz, Kunath Franziska, Gedrange Tomasz, Kunert-Keil Christiane
Department of Orthodontics, Carl Gustav Carus Campus, TU Dresden, Fetscherstrasse 74, 01307 Dresden, Germany.
Biomed Res Int. 2016;2016:5146285. doi: 10.1155/2016/5146285. Epub 2016 Aug 11.
The aim of this study was to examine the osteogenic potential of new flax covering materials. Bone defects were created on the skull of forty rats. Materials of pure PLA and PCL and their composites with flax fibers, genetically modified producing PHB (PLA-transgen, PCL-transgen) and unmodified (PLA-wt, PCL-wt), were inserted. The skulls were harvested after four weeks and subjected to histological examination. The percentage of bone regeneration by using PLA was less pronounced than after usage of pure PCL in comparison with controls. After treatment with PCL-transgen, a large amount of new formed bone could be found. In contrast, PCL-wt decreased significantly the bone regeneration, compared to the other tested groups. The bone covers made of pure PLA had substantially less influence on bone regeneration and the bone healing proceeded with a lot of connective tissue, whereas PLA-transgen and PLA-wt showed nearly comparable amount of new formed bone. Regarding the histological data, the hypothesis could be proposed that PCL and its composites have contributed to a higher quantity of the regenerated bone, compared to PLA. The histological studies showed comparable bone regeneration processes after treatment with tested covering materials, as well as in the untreated bone lesions.
本研究的目的是检测新型亚麻覆盖材料的成骨潜力。在40只大鼠的颅骨上制造骨缺损。将纯聚乳酸（PLA）、聚己内酯（PCL）及其与亚麻纤维的复合材料、经基因改造产生聚羟基丁酸酯的材料（PLA转基因材料、PCL转基因材料）以及未改性材料（PLA野生型材料、PCL野生型材料）植入其中。四周后取出颅骨并进行组织学检查。与对照组相比，使用PLA后的骨再生百分比不如使用纯PCL后明显。用PCL转基因材料处理后，可以发现大量新形成的骨。相比之下，与其他测试组相比，PCL野生型材料显著降低了骨再生。由纯PLA制成的骨覆盖物对骨再生的影响要小得多，骨愈合过程中有大量结缔组织，而PLA转基因材料和PLA野生型材料显示出几乎相当数量的新形成的骨。关于组织学数据，可以提出这样的假设：与PLA相比，PCL及其复合材料对再生骨的数量贡献更大。组织学研究表明，用测试的覆盖材料处理后以及在未处理的骨损伤中，骨再生过程具有可比性。
