Chen Liang, Xi Tingfei, Yang Zhiming, Wang Wei, Liu Qingyang, Wu Yingfeng, Gu Yongquan, Wang Jiancai, Feng Zengguo
Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Jun;19(6):481-5.
To investigate the effect of surface property of different polyether-ester block copolymers [poly(ethylene glycol-terephthalate)/poly(butylene terephthalate), PEGT/PBT] on the growth of smooth muscle cells (SMCs) and endothelial cells(ECs).
Three kinds of copolymers were synthesized, which were 1000-T20 (group A), 1000PEGT70/PBT30 (group B) and 600PEGT70/PBT30 (group C). The water-uptake and contact angle of three polyether-ester membranes were determined. The canine aorta smooth muscle cells and external jugular vein endothelial cells were primarily harvested, subcultured, and then identified. The proliferation of SMCs and ECs on the different polyether-ester membranes were investigated.
The water-uptake of three copolymers arranged as the sequence of group C<group A<group B, and contact angle as the sequence of group C>group A> group B, indicating group B being more hydrophilic. However, smooth muscle cells and endothelial cells grew poorly on the membrane of group B after low density seeding, but proliferated well on the membranes of group A and group C.
In contrast with more hydrophilic 1000PEGT70/PBT30, moderately hydrophilic 1000-T20 and 600PEGT70/PBT30 has better compatibility with vascular cells. The above results indicate that the vascular cells can grow well on moderately hydrophilic PEGT/PBT and that PEGT/PBT can be used in vascular tissue engineering.
研究不同聚醚酯嵌段共聚物[聚（乙二醇-对苯二甲酸酯）/聚（丁二醇-对苯二甲酸酯），PEGT/PBT]的表面性质对平滑肌细胞（SMCs）和内皮细胞（ECs）生长的影响。
合成了三种共聚物，分别为1000-T20（A组）、1000PEGT70/PBT30（B组）和600PEGT70/PBT30（C组）。测定了三种聚醚酯膜的吸水率和接触角。对犬主动脉平滑肌细胞和颈外静脉内皮细胞进行原代培养、传代培养，然后进行鉴定。研究了不同聚醚酯膜上SMCs和ECs的增殖情况。
三种共聚物的吸水率按C组<A组<B组的顺序排列，接触角按C组>A组>B组的顺序排列，表明B组更亲水。然而，低密度接种后，平滑肌细胞和内皮细胞在B组膜上生长较差，但在A组和C组膜上增殖良好。
与亲水性更强的1000PEGT70/PBT30相比，中等亲水性的1000-T20和600PEGT70/PBT30与血管细胞具有更好的相容性。上述结果表明，血管细胞能够在中等亲水性的PEGT/PBT上良好生长，且PEGT/PBT可用于血管组织工程。
