ARC Centre for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, University of Wollongong, Nth Wollongong, NSW 2500, Australia.
Acta Biomater. 2012 Jan;8(1):194-200. doi: 10.1016/j.actbio.2011.07.029. Epub 2011 Aug 2.
We have investigated the application of polypyrrole (pPy) as a material to influence neointimal cell behaviour. The physico-chemical properties of pPy doped with heparin (Hep), para-toluene sulfonate, poly(2-methoxyaniline-5-sulfonic acid) (pMAS) and nitrate ions were studied in addition to cell adhesion and proliferation studies of neointimal relevant cell lines cultured on the pPy substrates. Both smooth muscle (hSMC) and endothelial (hEC) cell types adhered and proliferated best on the smooth, hydrophilic pPy/pMAS material. Moreover, pPy/Hep is able to support the proliferation of hECs on the surface but inhibits hSMC proliferation after 4 days of culture. The inhibitory effect on hSMCs is most likely due to the well-known antiproliferative effect of heparin on hSMC growth. The results presented indicate that surface exposed heparin binds to the putative heparin receptor of hSMCs and is sufficient to inhibit proliferation. The application of galvanostatically synthesized pPy/Hep to stent surfaces presents a novel bioactive control mechanism to control neointimal cell growth.
我们研究了聚吡咯（pPy）作为一种材料对影响新生内膜细胞行为的应用。研究了肝素（Hep）、对甲苯磺酸盐、聚（2-甲氧基苯胺-5-磺酸）（pMAS）掺杂的 pPy 的物理化学性质以及培养在 pPy 基底上的新生内膜相关细胞系的细胞黏附和增殖研究。平滑肌（hSMC）和内皮细胞（hEC）在光滑亲水的 pPy/pMAS 材料上黏附和增殖最好。此外，pPy/Hep 能够支持 hEC 在表面上的增殖，但在培养 4 天后抑制 hSMC 的增殖。对 hSMC 的抑制作用很可能是由于肝素对 hSMC 生长的众所周知的抗增殖作用。所呈现的结果表明，暴露于表面的肝素结合到 hSMC 的肝素受体上，足以抑制增殖。将电化学合成的 pPy/Hep 应用于支架表面提供了一种新的生物活性控制机制来控制新生内膜细胞的生长。
