Deng Zhongxiang, Pang Yongzhen, Kong Weiwen, Chen Zhonghai, Wang Xinglong, Liu Xiaojun, Pi Yan, Sun Xiaofen, Tang Kexuan
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, 200433, P.R.China.
DNA Seq. 2005 Feb;16(1):28-35. doi: 10.1080/10425170500040180.
A new dehydrin ERD10 gene was cloned and characterized from Brassica napus (designated as Bndhn ERD10). The full-length cDNA of Bndhn ERD10 was 1114 bp and contained an open reading frame of 816 bp encoding a protein of 271 amino acid residues. The deduced Bndhn ERD10 protein contained an 8-serine residue domain and two conserved repeats of the characterized lysine-rich-K-segment (KIKEKLPG). Analysis of full-length cDNA and genomic DNA indicated that there were no introns in Bndhn ERD10 gene. The promoter of Bndhn ERD10 was further obtained by genomic walking technology, and analysis of the promoter indicated that the regulation of Bndhn ERD10 was ABA-dependent. Semi-quantitative RT-PCR of different tissues in unstressed B. napus plants indicated that the transcript of Bndhn ERD10 was more abundant in leaf than in stem and root. The expression profiles of Bndhn ERD10 in B. napus seedlings under various stress conditions including cold, salt and ABA were also investigated. Upon cold, salt and ABA stresses, increased transcript accumulations of the Bndhn ERD10 mRNAs were detected in young leaves 8 h after treatment.
从甘蓝型油菜中克隆并鉴定了一个新的脱水素ERD10基因（命名为Bndhn ERD10）。Bndhn ERD10的全长cDNA为1114 bp，包含一个816 bp的开放阅读框，编码一个由271个氨基酸残基组成的蛋白质。推导的Bndhn ERD10蛋白包含一个8-丝氨酸残基结构域和两个富含赖氨酸的特征性K片段（KIKEKLPG）的保守重复序列。全长cDNA和基因组DNA分析表明，Bndhn ERD10基因中没有内含子。通过基因组步移技术进一步获得了Bndhn ERD10的启动子，对启动子的分析表明Bndhn ERD10的调控是ABA依赖的。对未受胁迫的甘蓝型油菜植株不同组织进行半定量RT-PCR分析表明，Bndhn ERD10的转录本在叶片中比在茎和根中更丰富。还研究了Bndhn ERD10在甘蓝型油菜幼苗在包括冷、盐和ABA在内的各种胁迫条件下的表达谱。在冷、盐和ABA胁迫下，处理8小时后在幼叶中检测到Bndhn ERD10 mRNA的转录本积累增加。
