Mingeot Dominique, Dauchot Nicolas, Van Cutsem Pierre, Watillon Bernard
Biotechnology Department, Centre wallon de Recherches agronomiques, Chaussée de Charleroi 234, Gembloux, Belgium.
Mol Biol Rep. 2009 Sep;36(7):1995-2001. doi: 10.1007/s11033-008-9410-5. Epub 2008 Nov 8.
Two dehydrin genes were identified from a Cichorium intybus EST database. They were among the most abundant sequences obtained from 10 cDNA libraries constructed from chicory roots grown under field conditions. The full length cDNA sequences, designated CiDHN1 and CiDHN2, were 1,176 and 1,055 bp long and encoded predicted polypeptides of 262 and 261 amino acids, respectively. The deduced CiDHN1 protein contains a S-segment and four lysine-rich consensus motifs (K-segments) which represent a typical SK(4) structure of dehydrins. The CiDHN2 sequence contains two Y motifs and two K-segments classifying CiDHN2 as Y(2)K(2)-type dehydrin. Southern-blotting analysis suggested that CiDHN1 and CiDHN2 are single copy genes. Northern-blotting analysis revealed that both CiDHN genes are expressed in roots and leaves, with seasonal variations in transcript accumulation. The effect of cold on the CiDHN1 and CiDHN2 transcript level was demonstrated. CiDHN1 and CiDHN2 promoter analysis revealed the presence of low temperature-responsive and ABA-responsive elements.
从菊苣EST数据库中鉴定出两个脱水素基因。它们是从10个由田间条件下生长的菊苣根构建的cDNA文库中获得的最丰富的序列之一。全长cDNA序列，命名为CiDHN1和CiDHN2，长度分别为1176和1055 bp，分别编码预测的262和261个氨基酸的多肽。推导的CiDHN1蛋白包含一个S片段和四个富含赖氨酸的共有基序（K片段），它们代表脱水素的典型SK(4)结构。CiDHN2序列包含两个Y基序和两个K片段，将CiDHN2分类为Y(2)K(2)型脱水素。Southern杂交分析表明CiDHN1和CiDHN2是单拷贝基因。Northern杂交分析显示，两个CiDHN基因均在根和叶中表达，转录本积累存在季节性变化。证明了低温对CiDHN1和CiDHN2转录水平的影响。CiDHN1和CiDHN2启动子分析揭示了低温响应元件和ABA响应元件的存在。
