Wei Fang, Fan Yi, Zhang Min, Feng Yu-Qi
Department of Chemistry, Wuhan University, Wuhan, HuBei, P. R. China.
Electrophoresis. 2005 Aug;26(16):3141-50. doi: 10.1002/elps.200500043.
A method based on in-tube solid-phase microextraction and capillary zone electrophoresis (CZE) was proposed for simultaneously determining four amphetamines (amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, and 3,4-methylenedioxymethamphetamine) in urine. A poly(methacrylic acid-ethylene glycol dimethacrylate) monolithic capillary column, which can provide sufficient extraction efficiency, was introduced for the extraction of amphetamines from urine samples. The hydrophobic main chains and acidic pendant groups of the monolithic column make it a superior material for extraction of basic analytes from aqueous matrix. After extraction, the samples were analyzed by CZE. The best separation was achieved using a buffer composed of 0.1 M disodium hydrogen phosphate (adjusted to pH 4.5 with 1 M hydrochloric acid) and 20% methanol v/v, with a temperature and voltage of 25 degrees C and 20 kV, respectively. By applying electrokinetic injection with field-amplified sample stacking, detection limits of 25-34 microg/L were achieved. Excellent method of reproducibility was found over a linear range of 0.1-5 mg/L. Determination of these analytes from abusers' urine sample was also demonstrated.
提出了一种基于管内固相微萃取和毛细管区带电泳（CZE）的方法，用于同时测定尿液中的四种苯丙胺类物质（苯丙胺、甲基苯丙胺、3,4-亚甲基二氧基苯丙胺和3,4-亚甲基二氧基甲基苯丙胺）。引入了一种聚（甲基丙烯酸-乙二醇二甲基丙烯酸酯）整体毛细管柱，该柱可提供足够的萃取效率，用于从尿液样本中萃取苯丙胺类物质。整体柱的疏水主链和酸性侧基使其成为从水性基质中萃取碱性分析物的优质材料。萃取后，采用CZE对样品进行分析。使用由0.1 M磷酸氢二钠（用1 M盐酸调节至pH 4.5）和20%（v/v）甲醇组成的缓冲液，在温度为25℃、电压为20 kV的条件下实现了最佳分离。通过应用场放大进样的电动进样方式，检测限达到了25 - 34 μg/L。在0.1 - 5 mg/L的线性范围内发现了出色的重现性。还展示了对滥用者尿液样本中这些分析物的测定。
