Laboratory of Analytical Chemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Laboratory of Pharmaceutical Analysis, Department of Pharmaceutical Technology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Molecules. 2020 Apr 30;25(9):2096. doi: 10.3390/molecules25092096.
In-tube solid phase microextraction is a cutting-edge sample treatment technique offering significant advantages in terms of miniaturization, green character, automation, and preconcentration prior to analysis. During the past years, there has been a considerable increase in the reported publications, as well as in the research groups focusing their activities on this technique. In the present review article, HPLC bioanalytical applications of in-tube SPME are discussed, covering a wide time frame of twenty years of research reports. Instrumental aspects towards the coupling of in-tube SPME and HPLC are also discussed, and detailed information on materials/coatings and applications in biological samples are provided.
管内固相微萃取是一种前沿的样品处理技术，在微型化、绿色化、自动化和分析前浓缩方面具有显著优势。在过去的几年中，有关该技术的报道出版物以及专注于该技术的研究小组数量都有了相当大的增加。在本文综述中，讨论了管内 SPME 在 HPLC 生物分析中的应用，涵盖了 20 年来的研究报告。还讨论了管内 SPME 与 HPLC 耦合的仪器方面，并提供了有关材料/涂层的详细信息以及在生物样品中的应用。
