Huang Wei-yi
Guangxi University, Nanning 530004, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005 Apr 30;23(2):106-9.
OBJECTIVE To observe the characteristics of biology and pathogenicity of the third stage Anisakis simplex larvae (L3).
The survival time of the L3 in various conditions was observed and the pathological change after experimental infection in rats was examined.
The results showed that the L3 frozen at -20 degrees C for 10 - 12 h can be killed. In the temperature range of 4 - 10 degrees C, the L3, can survive for over 8 months. The L3 was very active at 37 degrees C, and was killed at the high temperature over 40 degrees C in a very short time. The ingredients for sashimi cannot kill the L3. The experiment of rats infected by the L3 showed that about 15% -25% of the L3 penetrated into the gastrointestinal wall or migrated into the peritoneal cavity in 2 days. After 3d the L4 was not infectious, and died automatically in 7 - 10 days and could not develop into adults. The animals can be easily infected when the stomach was empty. The pathological study showed that the primary infection was a kind of reaction to foreign body, while that of the re - infection was allergic.
The L3 has a strong resistance to low temperature and to ingredients, it can be killed by freezing at -20 degrees C in 24 hours. The L3 can not mature in the body of terrestrial mammals but causes pathological change in the stomach and allergy.
目的 观察简单异尖线虫三期幼虫（L3）的生物学特性和致病性。
观察L3在不同条件下的存活时间，并检测大鼠实验感染后的病理变化。
结果显示，L3在-20℃冷冻10 - 12小时可被杀死。在4 - 10℃温度范围内，L3可存活8个月以上。L3在37℃时非常活跃，在40℃以上高温下很快被杀死。生鱼片食材不能杀死L3。L3感染大鼠的实验表明，约15% - 25%的L3在2天内穿透胃肠壁或迁移至腹腔。3天后L4无感染性，7 - 10天内自动死亡，不能发育为成虫。胃排空时动物易被感染。病理研究表明，初次感染是一种对异物的反应，而再次感染是过敏反应。
L3对低温和食材有较强抵抗力，在-20℃冷冻24小时可被杀死。L3在陆生哺乳动物体内不能成熟，但可引起胃部病理变化和过敏反应。
