Tsuji Sumiko, Nakano Maki, Furukawa Mizuki, Yoshii Kimihiko S, Tonogai Yasuhide
Division of Food Chemistry, Osaka Branch, National Institute of Health Sciences, Japan.
Shokuhin Eiseigaku Zasshi. 2005 Jun;46(3):116-20. doi: 10.3358/shokueishi.46.116.
CFR and JECFA specify that the total color in FD&C Blue No. 2 (B2; Indigo Carmine, Indigotine, Food Blue No. 2) is not less than 85%, its isomer (B2iso) in B2 is not more than 18%, and its subsidiary color (B2sub) in B2 is not more than 2% (CFR) or 1% (JECFA). Japan's Specifications and Standards for Food Additives, 7th Edition, specifies that the total color in B2 is not less than 85.0% and other color materials in B2 are not detected by paper chromatography. LC/MS and HPLC were employed to identify and determine the main component (B2m) of B2, B2iso, and B2sub. The pseudo molecular ions (B2m and B2iso: [M-2Na+H]-, m/z=421; B2sub: [M-Na]-, m/z=341) of each color material were obtained and identified by LC/MS based on their absorptions and mass spectra. The contents of B2iso and B2sub in B2 samples (certified samples from fiscal year 1998 to fiscal year 2002) were determined by HPLC using calibration curves for the standards of B2m and B2iso. The contents of B2iso in most samples were less than 10%, and the contents of B2sub in all samples were not more than 1%. All of them were within the regulatory limits set by the CFR and JECFA.
美国联邦法规（CFR）和食品添加剂联合专家委员会（JECFA）规定，FD&C蓝色二号（B2；靛蓝胭脂红、靛蓝、食品蓝二号）的总色素含量不低于85%，B2中的异构体（B2iso）含量不超过18%，B2中的副色素（B2sub）含量不超过2%（CFR）或1%（JECFA）。日本《食品添加剂规格和标准》第7版规定，B2中的总色素含量不低于85.0%，且通过纸色谱法未检测到B2中的其他色素物质。采用液相色谱-质谱联用（LC/MS）和高效液相色谱（HPLC）对B2的主要成分（B2m）、B2iso和B2sub进行鉴定和测定。基于每种色素物质的吸收光谱和质谱，通过LC/MS获得并鉴定了它们的准分子离子（B2m和B2iso：[M - 2Na + H]-，m/z = 421；B2sub：[M - Na]-，m/z = 341）。使用B2m和B2iso标准品的校准曲线，通过HPLC测定了B2样品（1998财年至2002财年的认证样品）中B2iso和B2sub的含量。大多数样品中B2iso的含量低于10%，所有样品中B2sub的含量均不超过1%。所有这些含量均在CFR和JECFA规定的监管限值范围内。
