Office of Cosmetics and Colors, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, Maryland 20740, United States.
J Agric Food Chem. 2013 Apr 17;61(15):3726-36. doi: 10.1021/jf400029y. Epub 2013 Apr 9.
This study describes a new method for determining FD&C Blue No. 1, FD&C Blue No. 2, FD&C Green No. 3, FD&C Red No. 3, FD&C Red No. 40, FD&C Yellow No. 5, and FD&C Yellow No. 6 in food products. These seven color additives are water-soluble dyes that are required to be batch certified by the U.S. Food and Drug Administration (FDA) before they may be used in food and other FDA-regulated products. In the new method, the color additives are extracted from a product using one of two procedures developed for various product types, isolated from the noncolored components, and analyzed by liquid chromatography with photodiode array detection. The method was validated by determining linearity, range, precision, recovery from various matrices, limit of detection, limit of quantitation, and relative standard deviation for each color additive. A survey of 44 food products, including beverages, frozen treats, powder mixes, gelatin products, candies, icings, jellies, spices, dressings, sauces, baked goods, and dairy products, found total color additives ranging from 1.9 to 1221 mg/kg. FDA intends to use the new method for conducting a rigorous, comprehensive dietary exposure assessment of certified color additives in products likely to be consumed by children.
本研究描述了一种测定食品中七种食用色素（FD&C 蓝色素 1、FD&C 蓝色素 2、FD&C 绿色素 3、FD&C 红色素 3、FD&C 红色素 40、FD&C 黄色素 5 和 FD&C 黄色素 6）的新方法。这些七种色素添加剂均为水溶性染料，在美国食品药品监督管理局（FDA）批准其用于食品和其他受 FDA 监管的产品之前，必须对其进行批认证。在新方法中，通过为各种产品类型开发的两种程序之一从产品中提取色素添加剂，从非着色成分中分离出来，并通过带有光电二极管阵列检测的液相色谱法进行分析。该方法通过测定每种色素添加剂的线性、范围、精密度、从各种基质中的回收率、检测限、定量限和相对标准偏差进行了验证。对包括饮料、冷冻食品、粉末混合物、明胶产品、糖果、糖霜、果冻、香料、调味料、酱汁、烘焙食品和乳制品在内的 44 种食品进行的调查发现，总食用色素含量范围为 1.9 至 1221 毫克/千克。FDA 打算使用新方法对儿童可能食用的产品中经认证的食用色素进行严格、全面的饮食暴露评估。
