Hellsten Johan, West Mark J, Arvidsson Andreas, Ekstrand Joakim, Jansson Linda, Wennström Malin, Tingström Anders
Molecular Psychiatry Unit, Wallenberg Neuroscience Center, Lund, Sweden.
Biol Psychiatry. 2005 Dec 1;58(11):871-8. doi: 10.1016/j.biopsych.2005.05.023. Epub 2005 Jul 25.
Electroconvulsive seizure (ECS)-treatment, a model for electroconvulsive therapy (ECT) has been shown to induce proliferation of endothelial cells in the dentate gyrus (DG) of adult rats. Here we quantified the net angiogenic response after chronic ECS-treatment in the molecular layer (ML) of the dentate gyrus. Patients undergoing ECT are routinely oxygenated to prevent hypoxia, a known inducer of angiogenesis. Therefore we also examined the effect of oxygenation on ECS-induced proliferation of endothelial cells.
Total endothelial cell numbers and vessel length were estimated utilizing design based stereological analysis methods. Endothelial cell proliferation in the DG after ECS with or without oxygenation was assessed using bromodeoxyuridine.
The total number of endothelial cells and total vessel length was increased. Oxygenation did not abolish the ECS-induced proliferation of endothelial cells in the DG.
ECS-treatment induces a dramatic increase in endothelial cell proliferation leading to a 30% increase in the total number of endothelial cells. The increase in cell number resulted in a 16% increase in vessel length. These findings raise the possibility that similar vascular growth is induced by clinically administered ECT.
电惊厥发作（ECS）治疗作为电休克治疗（ECT）的一种模型，已被证明可诱导成年大鼠齿状回（DG）中的内皮细胞增殖。在此，我们对慢性ECS治疗后齿状回分子层（ML）中的净血管生成反应进行了量化。接受ECT治疗的患者通常会进行氧合以预防缺氧，而缺氧是一种已知的血管生成诱导因素。因此，我们还研究了氧合对ECS诱导的内皮细胞增殖的影响。
利用基于设计的体视学分析方法估计内皮细胞总数和血管长度。使用溴脱氧尿苷评估有或无氧合的ECS治疗后DG中的内皮细胞增殖情况。
内皮细胞总数和血管总长度增加。氧合并未消除ECS诱导的DG中内皮细胞的增殖。
ECS治疗可显著增加内皮细胞增殖，导致内皮细胞总数增加30%。细胞数量的增加使血管长度增加了16%。这些发现增加了临床应用ECT诱导类似血管生长的可能性。
