Hellsten Johan, Wennström Malin, Bengzon Johan, Mohapel Paul, Tingström Anders
Molecular Psychiatry Unit, Wallenberg Neuroscience Center, S-22184 Lund, Sweden.
Biol Psychiatry. 2004 Feb 15;55(4):420-7. doi: 10.1016/j.biopsych.2003.08.013.
Electroconvulsive seizures, an animal model for electroconvulsive treatment, induce a strong increase in neurogenesis in the dentate gyrus of adult rats. Hippocampal neurogenesis has previously been described as occurring in an angiogenic niche. This study examines the effect of electroconvulsive seizures on proliferation of vascular cells in rat hippocampus.
Rats were injected with bromodeoxyuridine to label proliferating cells in the dentate gyrus after single/multiple electroconvulsive seizures in a dose-response study and at various time points after single electroconvulsive seizures in a time-course study.
A dose-response effect on the number of bromodeoxyuridine-labeled endothelial cells located in the granule cell layer, hilus, and molecular layer was noted, as was the case with the number of neural precursors in the subgranular zone. The time-course study revealed that endothelial cell and neural precursor proliferation occurred in concert in response to a single electroconvulsive seizure.
Our data suggest that in response to electroconvulsive seizures, endothelial cell and neural proliferation is coregulated. The increase in endothelial cell proliferation may act to support the increased neural proliferation and neuronal activity or vice versa, possibly leading to structural changes within the hippocampus of importance for the antidepressant effect of electroconvulsive seizures.
电惊厥发作是电惊厥治疗的一种动物模型，可使成年大鼠齿状回中的神经发生显著增加。海马神经发生此前被描述为发生在血管生成微环境中。本研究考察电惊厥发作对大鼠海马中血管细胞增殖的影响。
在剂量反应研究中，大鼠单次/多次接受电惊厥发作后，以及在时程研究中，大鼠单次接受电惊厥发作后的不同时间点，注射溴脱氧尿苷以标记齿状回中的增殖细胞。
观察到位于颗粒细胞层、门区和分子层的溴脱氧尿苷标记的内皮细胞数量呈现剂量反应效应，颗粒下区的神经前体细胞数量也是如此。时程研究表明，单次电惊厥发作后，内皮细胞和神经前体细胞的增殖协同发生。
我们的数据表明，对电惊厥发作的反应中，内皮细胞和神经增殖是共同调节的。内皮细胞增殖的增加可能有助于支持神经增殖和神经元活动的增加，反之亦然，这可能导致海马体内发生对电惊厥发作的抗抑郁作用至关重要的结构变化。
