Williamson Anne E, Dawson Deborah V, Drake David R, Walton Richard E, Rivera Eric M
Department of Endodontics, College of Dentistry, University of Iowa, Iowa City, IA 52242, USA.
J Endod. 2005 Aug;31(8):599-604. doi: 10.1097/01.don.0000153843.25887.69.
Endotoxin, elaborated by gram-negative organisms, is an important factor in apical periodontitis. The objective of this study was to evaluate the magnitude of endotoxin penetration through root canal treated teeth using a dual chamber model system. Forty-four maxillary anterior teeth were prepared endodontically and canals filled either by lateral condensation or a warm thermoplasticized technique in combination with either Roth's 801 or AH 26 sealer. Teeth were suspended in the model system with a mixed anaerobic bacterial suspension in the upper chamber and HBSS in the lower chamber. The QCL-1000 LAL assay was used to measure endotoxin at 0, 1, 7, 14, and 21 days. Response feature analysis using trapezoidal area under the curve was performed; the four treatment groups were compared using nonparametric methods. Groups differed (p = 0.028), with thermoplasticized root canal filling/Roth's 801 sealer permitting the least apical endotoxin penetration. Results suggest that Roth's 801 sealer may have a role in inhibiting endotoxin penetration.
由革兰氏阴性菌产生的内毒素是根尖周炎的一个重要因素。本研究的目的是使用双腔模型系统评估内毒素通过根管治疗牙齿的渗透程度。44颗上颌前牙进行了根管预备，根管分别采用侧向加压法或热塑技术充填，并分别使用Roth's 801或AH 26封闭剂。牙齿悬浮于模型系统中，上腔为混合厌氧菌悬液，下腔为汉克斯平衡盐溶液（HBSS）。使用QCL-1000鲎试剂法在第0、1、7、14和21天测量内毒素。采用曲线下梯形面积进行反应特征分析；使用非参数方法比较四个治疗组。各组之间存在差异（p = 0.028），热塑根管充填/Roth's 801封闭剂导致的根尖内毒素渗透最少。结果表明，Roth's 801封闭剂可能在抑制内毒素渗透方面发挥作用。
