Zhang Zhong-Ming, Xie Zhao-Xia, Tan Da-Ren, Huang Cheng-Hui
Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Jun;30(3):292-4.
To explore the relationship among intracellular glutathione S-transferase activity (GST), the expression of lung resistance-related proteins (LRP) in acute leukemia, and its clinical effects.
The GST activity of bone marrow mononuclear cells and LRP expression in 57 acute leukemia patients were detected by the spectrophotometry assay and immuno-cytochemistry (SABC), respectively.
The GST activity of bone marrow mononuclear cells in the acute leukemia group was significantly higher than that of the control group (P < 0.01). The GST activity of mononuclear cells in acute leukemia was positively correlated with the percentage of blast in the bone marrow (r = 0.30, P < 0.05). The GST activity of mononuclear cells in the untreated acute leukemia group was obviously higher than that of the complete remission group (P <0.01). The GST activity in the refractory or relapsed acute leukemia group was significantly higher than that of the complete remission group and untreated leukemia group (P <0.05). In post-chemotherapy 13 of 17 the LRP-positive patients were the non-remission, 12 of the 20 LRP-negative patients were the complete remission. The curative rate of the LRP-positive group was the significantly lower than the LRP-negative group (P < 0.05). The GST activities of non-remission patients in the LRP-positive and LRP-negative group obviously increased.
The increase of GST activity in the bone marrow mononuclear cells is related to the clinical curative effects and the proliferation of blast in acute leukemia. Detection of LRP and GST activities in acute leukemia may have a reference value in judging the leukemia with drug resistance and estimating the prognosis.
探讨急性白血病细胞内谷胱甘肽S转移酶（GST）活性、肺耐药相关蛋白（LRP）表达及其临床意义。
采用分光光度法和免疫细胞化学法（SABC法）分别检测57例急性白血病患者骨髓单个核细胞GST活性和LRP表达。
急性白血病组骨髓单个核细胞GST活性明显高于对照组（P<0.01）。急性白血病单个核细胞GST活性与骨髓原始细胞比例呈正相关（r=0.30，P<0.05）。初治急性白血病组单个核细胞GST活性明显高于完全缓解组（P<0.01）。难治复发组GST活性明显高于完全缓解组和初治组（P<0.05）。化疗后LRP阳性患者中13例未缓解，LRP阴性患者中20例有12例完全缓解。LRP阳性组治愈率明显低于LRP阴性组（P<0.05）。LRP阳性和阴性组未缓解患者GST活性均明显升高。
急性白血病骨髓单个核细胞GST活性增高与临床疗效及原始细胞增殖有关。检测急性白血病LRP和GST活性对判断白血病耐药及估计预后有参考价值。
