Huang Bin-Tao, Xiao Zhen, Shi Yu-Tao, Ha Sen, Zhao Wei-Hong, Gao Da, Yan Xiao-Hong, Yang Hong
Department of Hematology, Affiliated Hospital, Inner Mongolia Medical College, Huhhot 010059, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Apr;15(2):262-6.
This study was purposed to investigate the relationship of expressions of gluthatione-S-transferase-pi (GST-pi), multidrug resistance protein-1 (MRP-1), lung resistance protein (LRP) with multidrug resistance of acute leukemia (AL), the correlation between 3 kinds protein expressions and the correlation of their protein expression with clinical features of AL patients. The S-P immunohistochemical staining method was used to determine the expressions of GST-pi, MRP1 and LRP proteins in 80 AL patients and 30 normal subjects. The results showed that there was the correlation between GST-pi, MRP1, LRP protein expression and chemotherapy resistance, meanwhile CR rates of patients with positive expression of those proteins were lower than that of patients with negative expression (P<0.05), so those protein expressions may be accounted for poor prognosis. There was the positive relationship between expression of GST-pi and MRP1 in refractory group (r=0.851, P<0.01). It is concluded that co-examination of GST-pi and MRP1 has greater significance than examination of one kind of protein in evaluating poor prognosis of leukemia patients. LRP protein expression increase obviously when WBC counts >or= 10 x 10(9)/L (63.6%, P<0.05), therefore LRP protein has great judging value for evaluating drug resistance and prognosis of acute leukemia patients whose peripheral blood WBC counts were high.
本研究旨在探讨谷胱甘肽-S-转移酶-π（GST-π）、多药耐药蛋白-1（MRP-1）、肺耐药蛋白（LRP）的表达与急性白血病（AL）多药耐药的关系，3种蛋白表达之间的相关性及其蛋白表达与AL患者临床特征的相关性。采用S-P免疫组化染色法检测80例AL患者和30例正常对照者GST-π、MRP1和LRP蛋白的表达。结果显示，GST-π、MRP1、LRP蛋白表达与化疗耐药相关，蛋白表达阳性患者的CR率低于阴性患者（P<0.05），提示这些蛋白表达可能是预后不良的原因。难治组GST-π与MRP1表达呈正相关（r=0.851，P<0.01）。结论：联合检测GST-π和MRP1较单项检测对评估白血病患者预后不良具有更大意义。当白细胞计数≥10×10⁹/L时LRP蛋白表达明显增加（63.6%，P<0.05），因此LRP蛋白对评估外周血白细胞计数高的急性白血病患者的耐药性及预后有重要判断价值。
