Gonçalves Alan G, Ducatti Diogo R B, Paranha Reinaldo G, Eugênia M, Duarte R, Noseda Miguel D
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Brazil.
Carbohydr Res. 2005 Sep 26;340(13):2123-34. doi: 10.1016/j.carres.2005.06.014.
Partial reductive hydrolysis was used to produce oligosaccharide alditols from repetitive sulfated galactans obtained from four Rhodophyta species: kappa-carrageenan (from Kappaphycus alvarezii), theta-carrageenan (Gigartina skottsbergii-alkali-treated lambda-carrageenan), agarose 6-sulfate (Gracilaria domingensis), and pyruvylated agarose 2-sulfate (Acanthophora spicifera-alkali-treated pyruvylated agaran sulfate). Each hydrolyzate was submitted to anion-exchange and gel-filtration chromatography, and the isolated oligosaccharide alditols were identified by 1D and 2D NMR spectroscopy and by ESI mass spectrometry. The positional isomers of the sulfated oligosaccharide alditols were then completely resolved by capillary electrophoresis in a borate buffer. Attempts to correlate the availability of the hydroxyl groups for borate complexation with the relative migration of the oligosaccharides are presented.
采用部分还原水解法，从四种红藻中提取的重复硫酸化半乳聚糖制备低聚糖糖醇：κ-卡拉胶（来自卡帕藻）、θ-卡拉胶（经碱处理的斯氏杉藻λ-卡拉胶）、6-硫酸化琼脂糖（多米尼加江蓠）和2-硫酸化丙酮酰化琼脂糖（经碱处理的具刺松节藻丙酮酰化琼脂糖硫酸酯）。每种水解产物都经过阴离子交换和凝胶过滤色谱法处理，分离得到的低聚糖糖醇通过一维和二维核磁共振光谱以及电喷雾电离质谱进行鉴定。然后，在硼酸盐缓冲液中通过毛细管电泳完全分离硫酸化低聚糖糖醇的位置异构体。本文还尝试将用于硼酸盐络合的羟基可用性与低聚糖的相对迁移率联系起来。
