Lamidi M, DiGiorgio C, Delmas F, Favel A, Eyele Mve-Mba C, Rondi M L, Ollivier E, Nze-Ekekang L, Balansard G
IPHAMETRA (Institute of Traditional Pharmacopoeia and Medicine), CENAREST, BP 842 Libreville, Gabon.
J Ethnopharmacol. 2005 Nov 14;102(2):185-90. doi: 10.1016/j.jep.2005.06.011. Epub 2005 Jul 19.
Seventy-seven crude extracts from leaves and stem barks of 15 Gabonese plants used in traditional medicine were evaluated for their cytotoxic, antileishmanial and antifungal activities. Most of the extracts exhibited cytotoxic activities toward human monocytes, and most particularly the hydromethanolic 50% (v/v) fraction of Ganophyllum giganteum leaves (IC(50)=1.3 microg/ml) as well as the methanolic extracts of Polyalthia suaveolens, Dioscorea preussii, Augouardia letestui leaves and Cola lizae stem barks (IC(50)<5 microg/ml). The methanolic extract of Polyalthia suaveolens displayed a strong antiproliferative activity against the promastigote form of Leishmania infantum parasites and presented a good antifungal activity on all the tested strains (IC(50)<1mg/ml). This extract was divided into six fractions: fraction F6 demonstrated a cytotoxic activity stronger than those of the crude extract (IC(50)=0.6 microg/ml), fractions F4 and F5 were devoid of cytotoxicity (IC(50)>100 microg/ml) and displayed interesting antileishmanial activity against the intracellular amastigote form of the parasite (IC(50)=5.6 and 12.4 microg/ml), respectively. However, the antifungal activity observed for the crude extract could not be recovered in the corresponding fractions.
对来自加蓬15种用于传统医学的植物的叶和茎皮的77种粗提物进行了细胞毒性、抗利什曼原虫和抗真菌活性评估。大多数提取物对人单核细胞表现出细胞毒性活性，尤其是大叶甘巴豆叶的50%（v/v）水甲醇提取物（IC50 = 1.3微克/毫升）以及平滑暗罗、普氏薯蓣、莱氏奥古豆叶和利氏可乐果茎皮的甲醇提取物（IC50 < 5微克/毫升）。平滑暗罗的甲醇提取物对婴儿利什曼原虫前鞭毛体形式显示出强烈的抗增殖活性，并对所有测试菌株表现出良好的抗真菌活性（IC50 < 1毫克/毫升）。该提取物被分为六个部分：F6部分表现出比粗提物更强的细胞毒性活性（IC50 = 0.6微克/毫升），F4和F5部分没有细胞毒性（IC50 > 100微克/毫升），并分别对寄生虫的细胞内无鞭毛体形式表现出有趣的抗利什曼原虫活性（IC50 = 5.6和12.4微克/毫升）。然而，在相应部分中未恢复粗提物所观察到的抗真菌活性。
