Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Phytother Res. 2010 Sep;24(9):1322-8. doi: 10.1002/ptr.3108.
The in vitro activity of the methanol extracts of 51 plants randomly collected from the Kingdom of Saudi Arabia and some of their fractions (petroleum ether, chloroform, ethyl acetate and aqueous) were evaluated against Plasmodium falciparum, Trypanosoma brucei brucei, T. cruzi and Leishmania infantum, as well as toxicity against MRC-5 fibroblast cells. Ten crude methanolic extracts that demonstrated potent and adequately selective antiprotozoal activity were subjected to solvent fractionation using petroleum ether, ethyl acetate and chloroform. Only three samples showed promising antiprotozoal activity. Argemone ochroleuca (CHCl(3) fraction) showed pronounced activity against P. falciparum(GHA) (IC(50) 0.32 microg/mL) and T. cruzi (IC(50) 0.30 microg/mL) with low cytotoxicity against MRC-5 cells (CC(50) 11.6 microg/mL). Capparis spinosa (EtOAc fraction) showed pronounced activity against P. falciparum(GHA) with an IC(50) 0.50 microg/mL in the absence of toxicity against MRC-5 cell line (CC(50) > 30 microg/mL). Heliotropium curassavicum (CHCl(3) fraction) showed similar activity against P. falciparum (IC(50) 0.65 microg/mL; MRC-5 CC(50) > 30 microg /mL). These three extracts will be subjected for further extensive studies to isolate and identify their active constituents.
从沙特阿拉伯王国随机采集的 51 种植物的甲醇提取物及其部分馏分（石油醚、氯仿、乙酸乙酯和水）对恶性疟原虫、布氏冈比亚锥虫、克氏锥虫和婴儿利什曼原虫进行了体外活性评估，同时还评估了它们对 MRC-5 成纤维细胞的毒性。对显示出有效且足够选择性抗原生动物活性的 10 种粗甲醇提取物进行了溶剂分级，使用了石油醚、乙酸乙酯和氯仿。只有三个样本显示出有希望的抗原生动物活性。罂粟（CHCl(3)馏分）对恶性疟原虫（GHA）（IC(50)0.32μg/mL）和克氏锥虫（IC(50)0.30μg/mL）表现出明显的活性，对 MRC-5 细胞的细胞毒性较低（CC(50)11.6μg/mL）。仙人掌（EtOAc 馏分）对恶性疟原虫（GHA）表现出明显的活性，IC(50)为 0.50μg/mL，对 MRC-5 细胞系无毒性（CC(50)>30μg/mL）。天芥菜（CHCl(3)馏分）对恶性疟原虫表现出类似的活性（IC(50)0.65μg/mL；MRC-5 CC(50)>30μg/mL）。这三种提取物将进一步进行广泛的研究，以分离和鉴定其活性成分。
