Pinkston J David
Procter & Gamble Pharmaceuticals, Health Care Research Center, 8700 Mason-Montgomery Rd, PO Box 8006, Mason, OH 45040, USA.
Eur J Mass Spectrom (Chichester). 2005;11(2):189-97. doi: 10.1255/ejms.731.
Mobile phases in supercritical fluid chromatography (SFC) have low viscosities and high diffusion coefficients with respect to those of traditional high performance liquid chromatography (HPLC). These properties allow higher mobile phase flow rates and/or longer columns in SFC, resulting in rapid analyses and high efficiency separations. In addition, chiral SFC is becoming especially popular. Mass spectrometry (MS) is arguably the most popular "informative" detector for chromatographic separations. Most SFC/MS is performed with atmospheric pressure ionization (API) sources. Unlike LC/MS, the interface between the SFC column and the API source must allow control of the downstream (post-column) pressure while also providing good chromatographic fidelity. Here we compare and contrast the popular interfacing approaches. Some are simple, such as direct effluent introduction with no active back-pressure-regulator (BPR) in high speed bioanalytical applications. The pressure-regulating-fluid interface is more versatile and provides excellent chromatographic fidelity, but is less user friendly. The pre-BPR- split interface and an interface which provides total-flow-introduction with a mechanical BPR are good compromises between user friendliness and performance, and have become the most popular among practitioners. Applications of SFC/MS using these various interfaces are also discussed.
与传统高效液相色谱法（HPLC）相比，超临界流体色谱法（SFC）中的流动相具有低粘度和高扩散系数。这些特性使得SFC能够采用更高的流动相流速和/或更长的色谱柱，从而实现快速分析和高效分离。此外，手性SFC正变得尤为流行。质谱（MS）可以说是色谱分离中最常用的“信息丰富型”检测器。大多数SFC/MS分析采用大气压电离（API）源。与LC/MS不同，SFC色谱柱与API源之间的接口必须能够控制下游（柱后）压力，同时还要保证良好的色谱保真度。在此，我们对几种常用的接口方法进行比较和对比。有些方法很简单，比如在高速生物分析应用中，直接引入流出物且不使用主动背压调节器（BPR）。压力调节流体接口则更加通用，能提供出色的色谱保真度，但对用户不太友好。柱前BPR分流接口以及采用机械BPR实现全流引入的接口，是在用户友好性和性能之间的良好折衷方案，已成为从业者中最受欢迎的接口。本文还讨论了使用这些不同接口的SFC/MS应用。
