黄体期血清游离DNA作为辅助生殖技术后妊娠失败的标志物。

Luteal phase serum cell-free DNA as a marker of failed pregnancy after assisted reproductive technology.

作者信息

Hart Elaine A, Patton William C, Jacobson John D, King Alan, Corselli Johannah, Chan Philip J

机构信息

Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, California 92350, USA.

出版信息

J Assist Reprod Genet. 2005 May;22(5):213-7. doi: 10.1007/s10815-005-4924-4.

DOI:10.1007/s10815-005-4924-4

PMID:16047583

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455499/

Abstract

PURPOSE

DNA-damaging factors have been reported in patients that failed to achieve pregnancy after assisted reproductive technologies (ART). The hypothesis was that increased circulating cell-free DNA released by damaged cells could predict unfavorable conditions leading to failed ART treatment. The objective was to compare the relative concentrations of cell-free DNA in the luteal phase sera of nonpregnant versus pregnant patients.

METHODS

Frozen-thawed sera (30 IVF cases) were obtained 1 week after embryo transfer. There were 16 pregnant and 14 nonpregnant cases and controls consisting of male sera (n = 8 cases). Modified isocratic capillary electrophoresis was performed and the images analyzed for cell-free DNA.

RESULTS

Circulating cell-free DNA were identified in the sera of all patients. The serum concentrations of high (12 kb) and low (1 kb) molecular weight cell-free DNA were similar for both nonpregnant and pregnant patients. Male control sera had higher cell-free DNA concentrations compared with females. Evaluation of sera from a control case showed no fluctuations in cell-free DNA concentrations throughout specific days of the menstrual cycle.

CONCLUSIONS

The results do not support the use of the luteal phase cell-free DNA concentration as a marker for failed pregnancies. The equal concentrations of high and low molecular weight cell-free DNA and ladder band-like gel patterns suggested cell apoptosis as the source of DNA.

摘要

目的

有报道称，在辅助生殖技术（ART）后未能成功怀孕的患者中存在DNA损伤因素。假设是受损细胞释放的循环游离DNA增加可预测导致ART治疗失败的不利情况。目的是比较未怀孕患者与怀孕患者黄体期血清中游离DNA的相对浓度。

方法

胚胎移植后1周获取冻融血清（30例体外受精病例）。有16例怀孕病例和14例未怀孕病例，对照组为男性血清（n = 8例）。进行改良等度毛细管电泳，并对图像进行游离DNA分析。

结果

在所有患者的血清中均鉴定出循环游离DNA。未怀孕和怀孕患者的高分子量（12 kb）和低分子量（1 kb）游离DNA的血清浓度相似。男性对照血清的游离DNA浓度高于女性。对一名对照病例的血清评估显示，在月经周期的特定日子里，游离DNA浓度没有波动。

结论

结果不支持将黄体期游离DNA浓度用作妊娠失败的标志物。高分子量和低分子量游离DNA浓度相等以及梯状条带样凝胶模式表明细胞凋亡是DNA的来源。

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