Sharma Prashant K, Gibcus Marjon J, van der Mei Henny C, Busscher Henk J
Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Groningen, the Netherlands.
Eur J Oral Sci. 2005 Aug;113(4):326-32. doi: 10.1111/j.1600-0722.2005.00220.x.
The presence and maturity of the salivary pellicle influences microbial adhesion and its tenacity in the oral cavity, posing a challenge to different plaque-control systems. Some plaque-control systems rely on surface-tension forces arising from passing microbubbles sprayed over the pellicle. Passage of such bubbles is accompanied by a high fluid flow, but systematic studies are lacking on the contribution of fluid flow vs. microbubbles towards plaque removal. Therefore, the aim of this study was to determine the detachment efficacy of laminar fluid flow (wall shear rates 11,000-16,000 s(-1)), with and without microbubbles, towards the detachment of Actinomyces naeslundii T14V-J1 and Streptococcus oralis J22, and their coadhering aggregates, from salivary pellicles formed over 2 h or 16 h from reconstituted human whole saliva. Microbubbles in a fluid flow were more efficient at inducing single bacterial detachment, resulting in almost complete (97%) removal for S. oralis J22 and a 15-fold increase in A. naeslundii T14V-J1 removal as compared to the detachment caused by fluid flow alone. A. naeslundii was more difficult to remove and apparently formed the strongest bonds with high-molecular-weight proteins in 16-h pellicles. The detachment of coaggregates after 2 min left a substantial amount of adhered bacterial mass, including more than 60% of singly attached A. naeslundii on the pellicle surface, providing nucleation sites for the de novo adhesion of coadhering streptococci.
唾液薄膜的存在及其成熟度会影响微生物在口腔中的黏附及其黏附强度，这给不同的牙菌斑控制体系带来了挑战。一些牙菌斑控制体系依赖于喷洒在薄膜上的微气泡所产生的表面张力。此类气泡通过时会伴随高流体流速，但关于流体流速与微气泡对牙菌斑清除的贡献，尚缺乏系统性研究。因此，本研究的目的是确定层流（壁面剪切速率为11,000 - 16,000 s(-1)）在有无微气泡情况下，对从重组人全唾液在2小时或16小时内形成的唾液薄膜上分离内氏放线菌T14V-J1、口腔链球菌J22及其共黏附聚集体的效果。流体流中的微气泡在诱导单个细菌分离方面更有效，与仅由流体流引起的分离相比，可使口腔链球菌J22的去除率几乎达到完全（97%），内氏放线菌T14V-J1的去除率提高15倍。内氏放线菌更难去除，且在16小时形成的薄膜中，它显然与高分子量蛋白质形成了最强的结合。2分钟后共聚集体的分离留下了大量黏附的细菌团块，包括薄膜表面上超过60%的单个附着的内氏放线菌，为共黏附链球菌的重新黏附提供了成核位点。
