Andrade M Amparo, Siles-Lucas Mar, López-Abán Julio, Carranza Cristina, Pérez-Arellano José L, Muro Antonio
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Spain.
Parasite Immunol. 2005 Jun;27(6):235-42. doi: 10.1111/j.1365-3024.2005.00774.x.
SUMMARY We investigated the in vitro effect of total excretory/secretory and somatic antigens from Ascaris suum adults (ESA and SA) and larvae 3 (ESL3 and SL3), and of 10 purified protein fractions from ESA components on rat alveolar macrophage nitric oxide (NO) production. Our results showed that in vitro incubation of macrophages with SA and SL3 antigens of A. suum did not result in NO release from cells, whereas incubation with ESA or ESL3 antigens resulted in the stimulation of NO production by these cells, both in a specific (inhibited by L-NAME and L-canavanine) and dose-dependent manner. In addition, we could demonstrate that a purified ESA fraction consisting of three Coomassie-stained bands of approximately 37, 44 and 46 kDa is involved in the in vitro triggering of NO production by host cells. These three bands were subjected to MALDI-peptide mass fingerprint, showing similarities with phosphoglycerate kinase, elongation factor Tu and enolase molecules, respectively. Future studies will focus on the characterization of these parasite-derived molecules.
摘要 我们研究了猪蛔虫成虫的总排泄/分泌抗原和体抗原（ESA和SA）以及三期幼虫的相应抗原（ESL3和SL3），以及ESA组分中的10种纯化蛋白组分对大鼠肺泡巨噬细胞一氧化氮（NO）产生的体外影响。我们的结果表明，体外培养巨噬细胞与猪蛔虫的SA和SL3抗原不会导致细胞释放NO，而与ESA或ESL3抗原孵育会导致这些细胞刺激NO产生，且呈特异性（被L-精氨酸甲酯和L-刀豆氨酸抑制）和剂量依赖性。此外，我们可以证明，一个由三条考马斯亮蓝染色带组成的纯化ESA组分，分子量约为37、44和46 kDa，参与宿主细胞体外触发NO产生。对这三条带进行了基质辅助激光解吸电离-肽质量指纹分析，结果显示它们分别与磷酸甘油酸激酶、延伸因子Tu和烯醇酶分子相似。未来的研究将集中于这些寄生虫衍生分子的特性研究。
