ASAP，一种双极纺锤体组装和胞质分裂所需的人类微管相关蛋白。
ASAP, a human microtubule-associated protein required for bipolar spindle assembly and cytokinesis.
作者信息
Saffin Jean-Michel, Venoux Magali, Prigent Claude, Espeut Julien, Poulat Francis, Giorgi Dominique, Abrieu Ariane, Rouquier Sylvie
机构信息
Institut de Génétique Humaine, Centre National de la Recherche Scientifique, Unité Propre de Recherche 1142, Rue de la Cardonille, 34396 Montpellier Cédex 5, France.
出版信息
Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11302-7. doi: 10.1073/pnas.0500964102. Epub 2005 Jul 27.
We have identified a unique human microtubule-associated protein (MAP) named ASAP for ASter-Associated Protein. ASAP localizes to microtubules in interphase, associates with the mitotic spindle during mitosis, localizes to the central body during cytokinesis and directly binds to purified microtubules by its COOH-terminal domain. Overexpression of ASAP induces profound bundling of cytoplasmic microtubules in interphase cells and aberrant monopolar spindles in mitosis. Depletion of ASAP by RNA interference results in severe mitotic defects: it provokes aberrant mitotic spindle, delays mitotic progression, and leads to defective cytokinesis or cell death. These results suggest a crucial role for ASAP in the organization of the bipolar mitotic spindle, mitosis progression, and cytokinesis and define ASAP as a key factor for proper spindle assembly.
我们鉴定出一种独特的人类微管相关蛋白（MAP），名为ASAP，即与星体相关的蛋白。ASAP在间期定位于微管，在有丝分裂期间与有丝分裂纺锤体相关联，在胞质分裂期间定位于中心体，并通过其COOH末端结构域直接结合纯化的微管。ASAP的过表达诱导间期细胞中细胞质微管的深度成束以及有丝分裂中的异常单极纺锤体。通过RNA干扰使ASAP缺失会导致严重的有丝分裂缺陷：它引发异常的有丝分裂纺锤体，延迟有丝分裂进程，并导致有缺陷的胞质分裂或细胞死亡。这些结果表明ASAP在双极有丝分裂纺锤体的组织、有丝分裂进程和胞质分裂中起关键作用，并将ASAP定义为正确纺锤体组装的关键因素。
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