Lejeune J M, Charachon R
Clinique Universitaire O.R.L. du CHU de Grenoble, France.
Acta Otolaryngol. 1992;112(2):174-9. doi: 10.1080/00016489.1992.11665399.
The aim of the study was to determine the usefulness of new immunobiological tests using fractioned human inner ear antigen in the investigation of 4 Menière's disease and 12 rapidly progressive sensorineural hearing loss (RPSNHL). Each patient underwent a full ENT examination and a full range of immunobiological tests comprising ESR, electrophoresis, IgG, A, M, circulating immune complexes, cryoglobulins, complement, organ and non-organ auto-antibodies. The two new tests were the lymphocyte transformation test (LTT) and the immunoperoxidase test against total or fractioned human inner ear antigens. Four main bands were found in electrophoresis with the following molecular weights: 130,000, 45,000, 22,000, and 14,000. The heaviest band corresponded to IgG contained in the inner ear extract. Three cases of bilateral RPSNHL had positive LTT against inner ear antigens. No serum of patients showed a characteristics band with the immunoperoxidase test with regard to the serum of healthy subjects. At least four abnormal inflammatory non-specific tests were shown in the cases of positive LTT. Out of 3, 2 patients received steroids and improved their hearing.
该研究的目的是确定使用人内耳抗原片段进行的新免疫生物学检测在4例梅尼埃病和12例快速进行性感音神经性听力损失（RPSNHL）研究中的效用。每位患者均接受了全面的耳鼻喉科检查以及一系列免疫生物学检测，包括血沉（ESR）、电泳、免疫球蛋白G、A、M、循环免疫复合物、冷球蛋白、补体、器官和非器官自身抗体。两项新检测为淋巴细胞转化试验（LTT）以及针对全人内耳抗原或人内耳抗原片段的免疫过氧化物酶试验。电泳中发现了四条主要条带，分子量如下：130,000、45,000、22,000和14,000。最重的条带对应于内耳提取物中含有的免疫球蛋白G。3例双侧RPSNHL患者的LTT对内耳抗原呈阳性。与健康受试者的血清相比，患者血清在免疫过氧化物酶试验中均未显示出特征性条带。LTT呈阳性的病例中至少有四项异常炎症非特异性检测结果。3例患者中有2例接受了类固醇治疗，听力得到改善。
