Verkerk A J, Eussen B H, Van Hemel J O, Oostra B A
Department of Clinical Genetics, University Hospital, Dijkzigt, The Netherlands.
Am J Med Genet. 1992;43(1-2):187-91. doi: 10.1002/ajmg.1320430131.
Cosmids, isolated from a 475 kb YAC that spans the fragile X region, and the YAC itself, were used for fluorescence in situ hybridization (FISH) on metaphase chromosomes from fragile X patients. Cosmid 22.3, containing most of the hybrid translocation breakpoints, shows in situ hybridization signals distal and proximal from the fragile X site. We propose that the size of the fragile site is limited to 20 kb.
