Brink H M, Slegers J F
Pflugers Arch. 1979 Dec;383(1):71-3. doi: 10.1007/BF00584477.
In perfusion studies on the isolated cellfree perfused rat kidney, the glomerular filtration rate (GFR) can be controlled if the GFR can be measured instantaneously. This paper reports on a GFR meter which opens this possibility. Vitamin B12 is used as a marker for the GFR. A bubble flow meter determines urine flow rates ranging from 10 up to 200 microliter-min-1. A fiberoptic colorimeter measures vitamin B12 concentrations in the urine up to 400 mg-l-1. The flow meter and the colorimeter are described in detail. The reliability of vitamin B12 as a marker for the GFR is demonstrated and, moreover, the identity of GFR values obtained with the GFR meter and those from B12 spectrophotometry is demonstrated.
在对离体无细胞灌注大鼠肾脏的灌注研究中，如果能即时测量肾小球滤过率（GFR），那么该滤过率就能得到控制。本文报道了一种使这成为可能的GFR测量仪。维生素B12被用作GFR的标志物。气泡流量计可测定范围为10至200微升/分钟的尿流速率。光纤比色计可测量尿液中高达400毫克/升的维生素B12浓度。文中详细描述了流量计和比色计。证明了维生素B12作为GFR标志物的可靠性，此外，还证明了用GFR测量仪获得的GFR值与通过B12分光光度法获得的值一致。
