不同血浆扩容剂无细胞灌注后离体大鼠肾脏的功能与形态（作者译）

[Function and morphology of isolated rat kidney following cellfree perfusion with various plasmaexpanders (author's transl)].

作者信息

Franke H, Sobotta E E, Witzki G, Unsicker K

出版信息

Anaesthesist. 1975 May;24(5):231-8.

PMID:1220580

Abstract

Isolated arteficially perfused rat kidneys prepared as described by Franke et al. (1971) were perfused for 60 min with solutions of Haemaccel, Dextran 40, Pluronic-F-108, or hydroxy-aethyl starch in a single pass system. The glomerular filtration rate (GFR) of the Haemaccel or Dextran 40 perfused organs amounted during the first 30 min to 0.58 ml X g-1 X min-1 and 0.47 ml X g-1 X min-1 respectively. Using Pluronic-F-108 or hydroxy-aethyl starch GFR rose to 0.94 ml X g-1 X min-1 and to 0.85 ml X G-1 X min-1. With Haemaccel or Dextran 40 solutions a mean tubular Na-reabsorption of 75.4 mumol X g-1 X min-1 and of 59 mumol X g-1 X min-1 respectively was determined. Employing Pluronic-F-108 or hydroxy-aethyl starch a mean sodium net transport of 92.6 mumol X g-1 X min-1 in both experimental groups was obtained. The differences described in the functional capabilities of Haemaccel or Dextran 40 and of Pluronic-F-108 or Hydroxyethyl starch perfused kidneys are in good accordance with morphological changes in the ultrastructure. The most striking morphological deviations were found in proximal tubules of those kidneys perfused with Haemaccel solutions. On the other hand after perfusion with hydroxyethyl starch only very few morphological alterations could be detected.

摘要

按照Franke等人（1971年）所述制备的离体人工灌注大鼠肾脏，在单通道系统中用贺斯、右旋糖酐40、普朗尼克-F-108或羟乙基淀粉溶液灌注60分钟。在最初30分钟内，用贺斯或右旋糖酐40灌注的器官的肾小球滤过率（GFR）分别为0.58 ml×g⁻¹×min⁻¹和0.47 ml×g⁻¹×min⁻¹。使用普朗尼克-F-108或羟乙基淀粉时，GFR分别升至0.94 ml×g⁻¹×min⁻¹和0.85 ml×g⁻¹×min⁻¹。使用贺斯或右旋糖酐40溶液时，测得的肾小管钠重吸收平均值分别为75.4 μmol×g⁻¹×min⁻¹和59 μmol×g⁻¹×min⁻¹。在两个实验组中，使用普朗尼克-F-108或羟乙基淀粉时，钠的平均净转运量为92.6 μmol×g⁻¹×min⁻¹。用贺斯或右旋糖酐40以及普朗尼克-F-108或羟乙基淀粉灌注的肾脏在功能能力上的差异与超微结构的形态学变化高度一致。在用贺斯溶液灌注的肾脏的近端小管中发现了最明显的形态学偏差。另一方面，用羟乙基淀粉灌注后，仅能检测到极少数形态学改变。