Barth Alfred, Siekel Peter, Sedlárová Eva, Valent Aladár, Tokhtaeva Elmíra
Food Research Institute, P.O. Box 25, Priemyselná 4, 824 75 Bratislava 26, Slovakia.
Acta Histochem. 2005;107(4):253-9. doi: 10.1016/j.acthis.2005.06.005. Epub 2005 Jul 27.
Watermelon cells after permeabilization in Tween 80 were immobilized by glutaraldehyde without any carrier. Cells immobilized by cross-linking demonstrated significantly lower aminopeptidase activities than untreated cells. Pectate and alginate hydrogels were used successfully for immobilization of watermelon cells which retained activities of some aminopeptidases. A simple and rapid procedure for determination of extracellular aminopeptidases was developed using a synthetic substrate. p-Nitroanilides of amino acids were used as substrates for the determination of the extracellular and intracellular enzymatic activities. The culture medium (without cells) was used for the identification and determination of extracellular enzyme activities, whereas intracellular activities were estimated from the cell suspension.
在吐温80中透化后的西瓜细胞在没有任何载体的情况下用戊二醛固定。通过交联固定的细胞显示出比未处理细胞显著更低的氨肽酶活性。果胶酸盐和藻酸盐水凝胶成功用于固定西瓜细胞，这些细胞保留了一些氨肽酶的活性。使用合成底物开发了一种简单快速的细胞外氨肽酶测定方法。氨基酸的对硝基苯胺用作测定细胞外和细胞内酶活性的底物。培养基（不含细胞）用于鉴定和测定细胞外酶活性，而细胞内活性则从细胞悬液中估算。
