Stano Ján, Siekel Peter, Micieta Karol, Barth Alfred
Food Research Institute, P.O. Box 25, Priemyselná 4, 824 75 Bratislava 26, Slovak Republic.
Acta Histochem. 2006;108(5):401-6. doi: 10.1016/j.acthis.2006.05.003. Epub 2006 Sep 12.
A simple, rapid and reproducible procedure for the identification and determination of extracellular saccharase from culture medium of watermelon cell suspension cultures is described. The culture medium (without cells) was used for the identification and determination of extracellular enzyme activity. Intracellular activity was estimated from the cell suspension. Watermelon cell suspension was permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest saccharase activity was at pH 4.6 at a temperature of 50 degrees C. The hydrolysis of substrate was linear 5h after reaching 60% conversion. The cells had high saccharase activity and good stability, and in long-term storage they showed convenient physico-mechanical properties.
本文描述了一种用于鉴定和测定西瓜细胞悬浮培养物培养基中胞外蔗糖酶的简单、快速且可重复的方法。使用培养基（不含细胞）来鉴定和测定胞外酶活性。从细胞悬浮液中估算细胞内活性。西瓜细胞悬浮液用吐温80透化处理，并用戊二醛固定。蔗糖酶活性最高时的pH值为4.6，温度为50摄氏度。底物水解在转化率达到60%后5小时呈线性。这些细胞具有高蔗糖酶活性和良好的稳定性，并且在长期储存中表现出便利的物理机械性能。
