Faganello Josiane, Arruda Walquíria, Schrank Augusto, Vainstein Marilene Henning
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, 91501-970, Porto Alegre, RS, Brazil.
J Microbiol Methods. 2006 Mar;64(3):416-9. doi: 10.1016/j.mimet.2005.07.003. Epub 2005 Jul 27.
In this work, an alternative to conventional preparation procedures for scanning electron microscopy (SEM) analysis of Cryptococcus neoformans was performed. The cells were fixed directly in the agar culture. This method is simpler than others already reported and the morphology of the cells was well preserved.
在这项工作中，我们对新型隐球菌扫描电子显微镜（SEM）分析的传统制备程序进行了改进。细胞直接在琼脂培养物中固定。该方法比已报道的其他方法更简单，并且细胞形态得到了很好的保存。
