Mata Juan, Marguerat Samuel, Bähler Jürg
Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
Trends Biochem Sci. 2005 Sep;30(9):506-14. doi: 10.1016/j.tibs.2005.07.005.
Gene expression is regulated at multiple levels, and cells need to integrate and coordinate different layers of control to implement the information in the genome. Post-transcriptional levels of regulation such as transcript turnover and translational control are an integral part of gene expression and might rival the sophistication and importance of transcriptional control. Microarray-based methods are increasingly used to study not only transcription but also global patterns of transcript decay and translation rates in addition to comprehensively identify targets of RNA-binding proteins. Such large-scale analyses have recently provided supplementary and unique insights into gene expression programs. Integration of several different datasets will ultimately lead to a system-wide understanding of the varied and complex mechanisms for gene expression control.
基因表达在多个层面受到调控，细胞需要整合并协调不同层次的控制，以实现基因组中的信息。转录后水平的调控，如转录本周转和翻译控制，是基因表达不可或缺的一部分，其复杂性和重要性可能与转录控制不相上下。基于微阵列的方法不仅越来越多地用于研究转录，还用于研究转录本降解的整体模式和翻译速率，此外还能全面鉴定RNA结合蛋白的靶标。此类大规模分析最近为基因表达程序提供了补充性的独特见解。整合几个不同的数据集最终将带来对基因表达控制的多样而复杂机制的全系统理解。
