血清及乳腺癌恶性肿瘤中的腺苷脱氨酶活性：同工酶ADA1和ADA2活性评估
Adenosine deaminase activity in the serum and malignant tumors of breast cancer: the assessment of isoenzyme ADA1 and ADA2 activities.
作者信息
Aghaei Mahmood, Karami-Tehrani Fatemeh, Salami Siamak, Atri Morteza
机构信息
Clinical Biochemistry Department, Cancer Research Laboratory, School of Medical Science, Tarbiat Modarres University, PO Box: 14115-111, Tehran, Iran.
出版信息
Clin Biochem. 2005 Oct;38(10):887-91. doi: 10.1016/j.clinbiochem.2005.05.015.
OBJECTIVE
The potential relationship between adenosine deaminase activity and cancer progression was examined by investigating the activity of total ADA and its isoenzymes in serum and simultaneously in the cancerous tissue of each patient with breast cancer.
METHODS
Total ADA and its isoenzymes were measured using the Giusti method. ADA2 activity was measured in the presence of a specific ADA1 inhibitor, EHNA.
RESULTS
Our results indicated that ADA2 and total ADA activities were higher in serum and malignant tissues than those of corresponding controls (P < 0.05). Tumor ADA2 and total ADA activities were significantly (P < 0.05) correlated with lymph node involvement, histological grade and tumor size, whereas their levels in serum were significantly (P < 0.05) correlated with menopausal status and patient age.
CONCLUSION
Although serum and tumor total ADA activity and its ADA2 isoenzyme were both found to be increased, distinct correlation patterns were observed with some of the prognostic factors. It can be speculated that increased ADA and isoenzyme activities in serum originated from sources other than the breast tumors.
目的
通过检测每位乳腺癌患者血清及癌组织中腺苷脱氨酶（ADA）的总活性及其同工酶活性，研究ADA活性与癌症进展之间的潜在关系。
方法
采用朱斯蒂法测定ADA总活性及其同工酶活性。在特异性ADA1抑制剂EHNA存在的情况下测定ADA2活性。
结果
我们的结果表明，血清和恶性组织中的ADA2及ADA总活性高于相应对照组（P < 0.05）。肿瘤ADA2及ADA总活性与淋巴结受累情况、组织学分级和肿瘤大小显著相关（P < 0.05），而其血清水平与绝经状态和患者年龄显著相关（P < 0.05）。
结论
虽然血清和肿瘤中的ADA总活性及其ADA2同工酶活性均升高，但与某些预后因素的相关性模式不同。可以推测，血清中ADA及同工酶活性升高并非源于乳腺肿瘤。
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