Kobayashi F, Ikeda T, Marumo F, Sato C
Second Department of Internal Medicine, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Am J Gastroenterol. 1993 Feb;88(2):266-71.
To clarify the clinical significance of increased serum adenosine deaminase (ADA) activity, and its mechanisms in various liver diseases, ADA isoenzyme activities (ADA1 and ADA2) in serum and the peripheral blood mononuclear cells were studied. High serum ADA activities were found in patients with acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis, liver cirrhosis, and hepatoma. The ADA2:ADA ratio was decreased in acute hepatitis, but was increased in chronic active hepatitis and liver cirrhosis. Clinically, ADA2 activity was correlated with serum gamma-globulin levels. In chronic active hepatitis, total ADA activities in the peripheral blood mononuclear cells were similar to those in controls. Furthermore, ADA2 activities after phytohemagglutinin (PHA) stimulation were significantly lower than those without PHA stimulation, although total ADA activities were increased after PHA stimulation. These findings suggest that serum ADA isoenzyme activities may be a new marker for liver disease, and that the increased serum ADA2 in chronic active hepatitis is unlikely to be the result of an increase in ADA2 production by activated peripheral blood mononuclear cells.
为阐明血清腺苷脱氨酶（ADA）活性升高在各种肝脏疾病中的临床意义及其机制，对血清及外周血单个核细胞中的ADA同工酶活性（ADA1和ADA2）进行了研究。在急性肝炎、酒精性肝纤维化、慢性活动性肝炎、肝硬化和肝癌患者中发现血清ADA活性升高。急性肝炎患者的ADA2:ADA比值降低，但慢性活动性肝炎和肝硬化患者的该比值升高。临床上，ADA2活性与血清γ-球蛋白水平相关。在慢性活动性肝炎中，外周血单个核细胞中的总ADA活性与对照组相似。此外，尽管在植物血凝素（PHA）刺激后总ADA活性增加，但PHA刺激后的ADA2活性显著低于未进行PHA刺激时。这些发现表明血清ADA同工酶活性可能是肝脏疾病的一个新标志物，且慢性活动性肝炎患者血清ADA2升高不太可能是活化的外周血单个核细胞产生ADA2增加的结果。
