Department of Biochemistry and Nutrition, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Clin Biochem. 2011 Aug;44(12):980-3. doi: 10.1016/j.clinbiochem.2011.05.020. Epub 2011 May 25.
To determine adenosine deaminase (ADA) activity as a possible diagnostic marker in HIV and HIV-HBV co-infected patients.
Blood samples were collected from 72 healthy, 33 HIV positive and 30 HIV-HBV co-infected subjects. Blood CD4+ cell count was recorded and serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total ADA, and ADA1 and ADA2 isoenzyme activities were determined.
Serum ALT, AST, total ADA and ADA2 isoenzyme activities were significantly higher in HIV positive and HIV-HBV co-infected groups compare to the control (p<0.05), whereas serum ALP showed no differences between groups. CD4+ cell counts markedly decreased in all patients and showed a significant inverse correlation with ADA activities (R(2)=0.589, p<0.001).
Serum ADA was significantly increased in HIV and HIV-HBV co-infections. Therefore, because of its low cost and simplicity to perform, ADA activity might be considered as a useful diagnostic tool among the other markers in these diseases.
确定腺苷脱氨酶 (ADA) 活性作为 HIV 和 HIV-HBV 合并感染患者的可能诊断标志物。
采集了 72 名健康人、33 名 HIV 阳性和 30 名 HIV-HBV 合并感染患者的血液样本。记录血液 CD4+细胞计数，并测定血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、碱性磷酸酶、总 ADA 以及 ADA1 和 ADA2 同工酶活性。
与对照组相比，HIV 阳性和 HIV-HBV 合并感染组的血清 ALT、AST、总 ADA 和 ADA2 同工酶活性显著升高（p<0.05），而血清 ALP 在各组之间无差异。所有患者的 CD4+细胞计数明显下降，与 ADA 活性呈显著负相关（R(2)=0.589，p<0.001）。
HIV 和 HIV-HBV 合并感染患者的血清 ADA 显著升高。因此，由于其成本低且操作简单，ADA 活性可能被认为是这些疾病中其他标志物的有用诊断工具。
